High-mobility Group Box 1 Facilitates CD4 T Cell Self-aggregation Via Integrin and STAT3 Activation Before Homing.

Inflammatory Bowel Diseases
Ying YuShuangjie Li

Abstract

High-mobility group box 1 (HMGB1) is one of the delayed pro-inflammatory cytokines produced in the later stages of pathogenesis and plays an important role in the progression of various inflammatory and autoimmune diseases. High-mobility group box 1 is able to stimulate interaction between integrins and cell adhesion molecules to facilitate cell-cell aggregation in "tissue-specific" endothelium; however, whether and how HMGB1 affects the adhesive capability of early acting immune cells in bloodstream remains largely unknown. Human peripheral blood samples were collected from healthy adult donors. The CD4 T cells were isolated from blood using CD4 T cell isolation kit and identified using flow cytometry and immunofluorescence staining. The effect of HMGB1 on adhesive ability of CD4 T cells was accessed by cell self-aggregation assay and endothelial adhesion assay. The migratory ability of CD4 T cells was evaluated by cell migration assay. Secretion of pro-inflammatory cytokines or chemokine C-X-C motif chemokine 12 (CXCL12) were detected by ELISA. Expression of integrins β1, β7, and α4β7 were determined by flow cytometric analysis. Inhibition of integrins was achieved with anti-integrin antibodies or cyclic peptide inhibitors. A...Continue Reading

References

Aug 14, 1999·Science·F G Giancotti, E Ruoslahti
Sep 8, 2000·The Journal of Biological Chemistry·L BarberisF G Giancotti
May 26, 2004·Biochemical Society Transactions·D A Calderwood
Jan 28, 2005·Inflammation·Klaus ZimmermannFriedrich Scheiflinger
Apr 2, 2005·Nature Reviews. Immunology·Michael T Lotze, Kevin J Tracey
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ingrid E DumitriuPatrizia Rovere-Querini
Jan 16, 2007·Circulation Research·Emmanouil ChavakisStefanie Dimmeler
Mar 3, 2010·Annual Review of Immunology·Gary P SimsAnthony J Coyle
Sep 10, 2010·American Journal of Physiology. Cell Physiology·Arnaud FriggeriEdward Abraham
Jul 27, 2011·The American Journal of Gastroenterology·Roberta VitaliSalvatore Cucchiara
Sep 15, 2011·PloS One·Alessandra CiucciGiorgio Mancino
Feb 3, 2015·The Journal of Clinical Investigation·Xiaorong ZhuEugene B Chang
Jul 25, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Eun-Ju YangJong-Sup Bae
Dec 10, 2015·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·G LiH D Zou
Mar 28, 2017·International Journal of Molecular Sciences·Agnieszka Irena Mazur-BialyMarcin Zarawski
Jan 26, 2018·Science Translational Medicine·Aida SivroUNKNOWN CAPRISA004 and RV254 study groups
May 3, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zhenggang LuanAiping Wang
Jan 27, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Stephanie L C ScofieldKrishna Singh

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