High mobility group box 1 promotes the epithelial-to-mesenchymal transition in prostate cancer PC3 cells via the RAGE/NF-κB signaling pathway

International Journal of Oncology
Jingliang ZhangWeijun Qin

Abstract

High mobility group box 1 (HMGB1), a critical damage-associated molecular pattern molecule, has been implicated in several inflammatory diseases and cancer types. The overexpression of HMGB1 protein occurs in prostate cancer, and is closely associated with the proliferation and aggressiveness of tumor cells. However, the underlying mechanisms of HMGB1-induced tumor metastasis in prostate cancer remain unclear. In the present study, it was demonstrated that the expression of HMGB1 was high in prostate cancer samples, particularly in the metastatic tissues. Furthermore, recombinant HMGB1 (rHMGB1) enhanced the invasive and metastatic capabilities of the prostate cancer cells. Molecular phenotype alterations of epithelial-to-mesenchymal transition (EMT) and elevated expression levels of matrix metalloproteinase (MMP)-1, -3 and -10 were observed. In addition, advanced glycosylation end-product specific receptor (RAGE) and its downstream molecule nuclear factor (NF)-κB pathway were activated during rHMGB1-induced metastasis. Silencing RAGE or NF-κB reversed the upregulation of MMP and EMT marker expression levels, thus reducing the migration and invasiveness of tumor cells. Taken together, these results suggest that highly expressed ...Continue Reading

Citations

Mar 7, 2020·The Kaohsiung Journal of Medical Sciences·Chun-Ping SongMing-Zhen Wang
Mar 17, 2020·Investigative Ophthalmology & Visual Science·Christelle GrossVincent Sapin
Jul 16, 2020·Cells·Bernardo L RapoportRonald Anderson
Jun 2, 2020·Frontiers in Oncology·Deborah P Lavin, Vijay K Tiwari
Sep 6, 2019·Oxidative Medicine and Cellular Longevity·Nurbubu T MoldogazievaAlexander A Terentiev
May 24, 2020·International Journal of Molecular Sciences·Ali H El-FarShaker A Mousa
Oct 8, 2020·International Journal of Environmental Research and Public Health·Ying-Erh ChouShun-Fa Yang
Oct 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eun Yeong LimMin Jung Kim

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Methods Mentioned

BETA
biopsy
protein assay
transgenic

Software Mentioned

Fiji
GraphPad
ImageJ
GraphPad Prism

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