High-mobility group box-1 translocation and release after hypoxic ischemic brain injury in neonatal rats

Experimental Neurology
Xiaodi ChenBarbara S Stonestreet

Abstract

Inflammation contributes to neonatal brain injury. Pro-inflammatory cytokines represent key inflammatory meditators in neonatal hypoxic-ischemic (HI) brain injury. The high mobility group box-1 (HMGB1) protein is a nuclear protein with pro-inflammatory cytokine properties when it is translocated from the nucleus and released extracellularly after stroke in adult rodents. We have previously shown that HMGB1 is translocated from the nucleus to cytosolic compartment after ischemic brain injury in fetal sheep. In the current study, we utilized the Rice-Vannucci model to investigate the time course of HMGB1 translocation and release after HI injury in neonatal rats. HMGB1 was located in cellular nuclei of brains from sham control rats. Nuclear to cytoplasmic translocation of HMGB1 was detected in the ipsilateral-HI hemisphere as early as zero h after HI, and released extracellularly as early as 6 h after HI. Immunohistochemical double staining detected HMGB1 translocation mainly in neurons along with release from apoptotic cells after HI. Serum HMGB1 increased at 3 h and decreased by 24 h after HI. In addition, rat brains exposed to hypoxic injury alone also exhibited time dependent HMGB1 translocation at 3, 12 and 48 h after hypoxi...Continue Reading

Citations

Jul 6, 2019·Journal of Neuropathology and Experimental Neurology·Adriel Barrios-AndersonBarbara S Stonestreet
Dec 5, 2019·Journal of Neuroscience Research·Boram KimBarbara S Stonestreet
Jul 3, 2020·International Journal of Molecular Sciences·Yam Nath PaudelMohd Farooq Shaikh
Nov 4, 2020·Immunity, Inflammation and Disease·Bo LiKai Le
Jan 2, 2021·Frontiers in Cellular Neuroscience·Xiaoyun GouDezhi Mu
Feb 10, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kazuki HatayamaBarbara S Stonestreet
Dec 17, 2020·Cells·Masahiro NishiboriHidenori Wake
Mar 2, 2021·Neural Regeneration Research·Kazuki Hatayama, Barbara S Stonestreet
Apr 2, 2021·Scientific Reports·Bernadette C BakerRebecca L Jones
Sep 30, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kathryn A Schiel

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