High mobility group box 2 regulates skeletal muscle development through ribosomal protein S6 kinase 1.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Ying FangDelin Mo

Abstract

HMGB2, a DNA-binding protein, highly expresses during embryogenesis and plays an important role in development of some organs and tissues. However, it remains to be further investigated weather HMGB2 influences muscle development. In this work, we identified HMGB2 as an essential factor in myogenesis. Compared to wild type (WT) mice, body weights of systemic hmgb2 homozygous knockout (hmgb2-/- ) mice especially males were reduced. Diameter and cross-section area of tibialis anterior (TA) muscle fibers as well as expression of Myogenin and MyHC were all decreased in hmgb2-/- mice. CTX injury model revealed that HMGB2 was required for satellite cell proliferation and muscle regeneration. Moreover, HMGB2 interacted with S6K1 and regulated the kinase activity of S6K1 during cell proliferation. Knockdown and inactivation of S6K1 in C2C12 cells both resulted in impaired proliferation and differentiation. Furthermore, expression of cyclin D1 and Myf5 were both decreased when HMGB2 or S6K1 were knocked down and kinase activity of S6K1 was inhibited. These results indicate that HMGB2 is required for skeletal muscle development and regeneration, and HMGB2 maintains proliferation of myoblasts through regulating kinase activity of S6K1.

References

Jan 1, 1992·Cell and Tissue Research·M D GroundsM W Beilharz
Jan 26, 2002·Journal of Muscle Research and Cell Motility·Z Zhou, A Bornemann
Feb 18, 2003·Journal of Anatomy·Margaret BuckinghamFrédéric Relaix
Sep 24, 2004·Nature·Lina Kassar-DuchossoyShahragim Tajbakhsh
Oct 2, 2009·American Journal of Physiology. Cell Physiology·Yejing GeJie Chen
Feb 4, 2010·Biochimica Et Biophysica Acta·Michal Stros
Dec 16, 2010·Nature Reviews. Molecular Cell Biology·Roberto ZoncuDavid M Sabatini
Sep 6, 2011·The Journal of Biological Chemistry·Noboru TaniguchiMartin Lotz
Feb 4, 2012·Cold Spring Harbor Perspectives in Biology·C Florian BentzingerMichael A Rudnicki
Mar 1, 2012·Journal of Cell Science·Barbara Gayraud-MorelShahragim Tajbakhsh
Sep 25, 2012·Nucleic Acids Research·Clemens Bönisch, Sandra B Hake
Mar 16, 2013·Stem Cells·Pearl A Campbell, Michael A Rudnicki
Mar 31, 2015·Life Sciences·Mariana R TavaresFernando M Simabuco
Jan 29, 2016·Nucleic Acids Research·Amaresh C PandaMyriam Gorospe
Sep 28, 2016·Journal of Cell Science·Xingyu ZhouDelin Mo
Oct 6, 2016·Cell Reports·Manuela MarabitaBert Blaauw
Jan 29, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Xin YiJiang Chang
Jan 9, 2018·Experimental Biology and Medicine·Hasan A AsfourRaed S Said
Nov 30, 2018·Circulation Journal : Official Journal of the Japanese Circulation Society·Michio SatoYuichi Oike
May 11, 2019·Proceedings of the National Academy of Sciences of the United States of America·Chintan K KikaniJared Rutter
Sep 10, 2019·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shuangfei NiJianzhong Hu

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