PMID: 9161031Apr 1, 1997Paper

High mobility group proteins HMG-1 and HMG-I/Y bind to a positive regulatory region of the pea plastocyanin gene promoter

The Plant Journal : for Cell and Molecular Biology
C I WebsterJ C Gray

Abstract

A 268 bp region (P268) of the pea plastocyanin gene promoter responsible for high-level expression has been shown to interact with the high mobility group proteins HMG-1 and HMG-I/Y isolated from pea shoot chromatin. cDNAs encoding an HMG-1 protein of 154 amino acid residues containing a single HMG-box and a C-terminal acidic tail and an HMG-I/Y-like protein of 197 amino acid residues containing four AT-hooks have been isolated and expressed in Escherichia coli to provide large amounts of full-length proteins. DNase I footprinting identified eight binding sites for HMG-I/Y and six binding sites for HMG-1 in P268. Inhibition of binding by the antibiotic distamycin, which binds in the minor groove of A/T-rich DNA, revealed that HMG-I/Y binding was 400-fold more sensitive than HMG-1 binding. Binding-site selection from a pool of random oligonucleotides indicated that HMG-I/Y binds to oligonucleotides containing stretches of five or more A/T bp and HMG-1 binds preferentially to oligonucleotides enriched in dinucleotides such as TpT and TpG.

Citations

Nov 14, 2000·Plant Science : an International Journal of Experimental Plant Biology·T MasekP M Pechan
Aug 12, 2003·Gene·Qiang WuPrakash P Kumar
Jun 11, 1999·The Plant Journal : for Cell and Molecular Biology·J F Martínez-García, P H Quail
Jul 16, 1999·The Plant Journal : for Cell and Molecular Biology·D Hatton, J C Gray
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·K RöttgersK D Grasser
Jun 8, 2001·European Journal of Biochemistry·C I WebsterJ C Gray
Feb 14, 1998·European Journal of Biochemistry·C StemmerK D Grasser
Jul 11, 2012·BMC Plant Biology·Junhua LiChangbin Chen
Apr 5, 2013·PLoS Computational Biology·Philip StegmaierJürgen Borlak
Sep 4, 2014·The Plant Journal : for Cell and Molecular Biology·Takafumi MidorikawaKentaro Inoue
Jul 1, 2003·Molecular Plant Pathology·Steven J KlostermanLee A Hadwiger
Mar 26, 2003·Biochemistry·Christian StemmerKlaus D Grasser
Jun 19, 2001·Biochimica Et Biophysica Acta·R Reeves, L Beckerbauer
Apr 28, 2004·Biochemical and Biophysical Research Communications·Jacek LichotaKlaus D Grasser
Mar 28, 2006·Journal of Molecular Biology·Marion GrasserKlaus D Grasser
Feb 24, 2007·Biochimica Et Biophysica Acta·Klaus D GrasserMarion Grasser
Jul 30, 2008·Biochemical and Biophysical Research Communications·Carina LyngaardKlaus D Grasser

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