PMID: 8943153Nov 12, 1996Paper

High molecular mass agarose matrix supports growth of bloodstream forms of pleomorphic Trypanosoma brucei strains in axenic culture

Molecular and Biochemical Parasitology
Erik Vassella, M Boshart

Abstract

Primary axenic culture of Trypanosoma brucei bloodstream forms almost invariably requires a period of culture adaptation with cell death and clonal selection. This has been particularly difficult and in many cases unsuccessful for natural pleomorphic strains, which are characterized by their ability to differentiate from replicating long slender bloodstream forms into short stumpy forms. Here we show that a representative set of pleomorphic T. brucei strains can be cultured in vitro on agarose plates without any previous adaptation period and selection. The slender morphology was retained and the growth rate was identical to the growth rate in vivo. Long term in vitro culture for 3 months with this method did not affect the ability of the AnTat 1.1 strain to give rise to pleomorphic infections in mice. Stumpy populations emanating from these infections transformed rapidly and synchronously into dividing procyclic forms when triggered with cis-aconitate and a temperature shift. The growth supporting activity of agarose plates could be traced to high molecular mass polymeric agarose; beta-agarase digestion destroyed the activity. Membrane chamber experiments show that direct physical contact of trypanosomes with the agarose matri...Continue Reading

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Citations

Jun 8, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Paula MacGregor, Keith R Matthews
Oct 1, 2013·Molecular and Biochemical Parasitology·Sebastian Knüsel, Isabel Roditi
Jan 5, 2002·Molecular and Biochemical Parasitology·Peter BütikoferIsabel Roditi
Mar 13, 1998·Molecular and Biochemical Parasitology·B ReunerM Boshart
Mar 13, 1998·Molecular and Biochemical Parasitology·E VassellaM Boshart
May 9, 1998·Molecular and Biochemical Parasitology·G A CrossM Navarro
May 11, 1999·Parasitology Today·K R Matthews
Sep 8, 1999·The Biochemical Journal·B FastD Steverding
Nov 14, 2002·Molecular Biology of the Cell·Ingrid B MüllerErik Vassella
Apr 11, 2003·Molecular Biology of the Cell·Erik VassellaIsabel Roditi
Jul 13, 2006·Eukaryotic Cell·Debora Domenicali PfisterErik Vassella
Apr 27, 2013·Molecular Microbiology·Gabriela Schumann BurkardIsabel Roditi
May 4, 1999·Memórias do Instituto Oswaldo Cruz·G Rudenko
Sep 6, 2001·The Journal of Parasitology·J R Seed, S J Black
Oct 1, 2003·The Journal of Biological Chemistry·Anne-Catherine SchlaeppiPeter Bütikofer
May 7, 2016·PloS One·Emma R ColdJosé A Fernández Robledo
Feb 4, 2003·The Journal of Biological Chemistry·Susanne W H van WeeldenMichael Boshart
Jul 14, 2019·Scientific Reports·David ChiassonMartin Parniske
Apr 29, 2021·PLoS Neglected Tropical Diseases·Federico RojasKeith R Matthews
Aug 5, 2021·Annual Review of Microbiology·Keith R Matthews

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