DOI: 10.1101/490920Dec 10, 2018Paper

High-molecular-weight polymers from dietary fiber drive aggregation of particulates in the murine small intestine

BioRxiv : the Preprint Server for Biology
Asher Preska SteinbergRustem F Ismagilov

Abstract

The lumen of the small intestine (SI) is filled with particulates: microbes, therapeutic particles, and food granules. The structure of this particulate suspension could impact uptake of drugs and nutrients and the function of microorganisms; however, little is understood about how this suspension is re-structured as it transits the gut. Here, we demonstrate that particles spontaneously aggregate in SI luminal fluid ex vivo. We find that mucins and immunoglobulins are not required for aggregation. Instead, aggregation can be controlled using polymers from dietary fiber in a manner that is qualitatively consistent with polymer-induced depletion interactions, which do not require specific chemical interactions. Furthermore, we find that aggregation is tunable; by feeding mice dietary fibers of different molecular weights, we can control aggregation in SI luminal fluid. This work suggests that the molecular weight and concentration of dietary polymers play an underappreciated role in shaping the physicochemical environment of the gut.

Related Concepts

Diet
Dietary Fiber
Environment
Food
Immunoglobulin G
Intestines, Small
Laboratory mice
Mucins
Mus
Polymers

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Biochemical Society Transactions
J M Creeth, B Cooper
Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists
L A Tabak
© 2021 Meta ULC. All rights reserved