High mucosal levels of tumor necrosis factor alpha messenger RNA in AIDS-associated cytomegalovirus-induced esophagitis

Gastroenterology
C M WilcoxP D Smith

Abstract

To evaluate the role of tumor necrosis factor alpha (TNF-alpha), a key inflammatory cytokine, in cytomegalovirus-associated gastrointestinal disease, we quantitated the level of TNF-alpha messenger RNA (mRNA) in esophageal mucosa from patients with cytomegalovirus-associated esophagitis and acquired immunodeficiency syndrome. Four patients underwent endoscopic biopsy of their cytomegalovirus-associated esophageal ulcers before and after ganciclovir therapy. The level of TNF-alpha mRNA in coded esophageal specimens was assessed by in situ hybridization, reverse-transcription polymerase chain reaction, and quantitative polymerase chain reaction. Esophageal mucosa from 3 patients whose ulcers healed or markedly improved contained before therapy numerous macrophages expressing TNF-alpha mRNA and high tissue levels of TNF-alpha mRNA that decreased substantially or were not detectable after therapy. In contrast, esophageal specimens from the single patient whose ulcer worsened after therapy contained many mucosal macrophages expressing TNF-alpha mRNA before as well as after therapy, and the high number of molecules of TNF-alpha mRNA present in the tissue before therapy increased further after treatment. Increased macrophage productio...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A CarswellB Williamson
Nov 1, 1992·The Journal of Clinical Investigation·P D SmithS M Wahl
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A M WangD F Mark
Oct 24, 1986·Science·K J TraceyJ D Albert
Jul 1, 1994·Gastrointestinal Endoscopy·C M WilcoxD A Schwartz
Nov 1, 1993·Annals of Internal Medicine·R W Goodgame
Jun 27, 1996·The New England Journal of Medicine·F Bazzoni, B Beutler

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Citations

Sep 12, 2000·Current Gastroenterology Reports·R W Goodgame
Dec 2, 2000·Molecular Medicine Today·R C Pollok, M J Farthing
Jan 13, 2000·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·S J WalkerN Birbeck
Oct 7, 2006·Current Opinion in Gastroenterology·G Meng, P D Smith
Feb 28, 2002·The American Journal of Gastroenterology·Paul R HarrisPhillip D Smith
Oct 24, 2006·Journal of Leukocyte Biology·Akhil MaheshwariPhillip D Smith
Feb 15, 2019·Frontiers in Immunology·Mathew Clement, Ian R Humphreys
May 1, 2021·International Journal of Molecular Sciences·Edoardo TronconeGiovanni Monteleone

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