PMID: 7542225Apr 21, 1995Paper

High nm23-H1/NDPK-A expression in Ewing tumors: paradoxical immunohistochemical reactivity and lack of prognostic significance

International Journal of Cancer. Journal International Du Cancer
D N AryeeH Kovar

Abstract

Expression of nm23-H1/NDPK-A has been reported to correlate inversely with metastasizing potential of rodent experimental cells and some human tumors. In the search for reliable molecular prognostic indicators for Ewing tumors (ET), a group of aggressive presumably neuroectodermal malignancies in children and adolescents, we studied nm23-H1/NDPK-A expression. Northern-blot and RT-PCR analyses were employed to semi-quantificatively measure nm23-H1 mRNA levels in ET cell lines and tissue extracts. A panel of monoclonal antibodies (MAbs) were used to evaluate protein abundance by Western blotting and immunohistochemistry. The nm23-H1/NDPK-A gene was also investigated on the DNA level to define possible genomic alterations. Our results revealed neither nm23-H1 allelic loss nor gene amplification and failed to show any significant variation in nm23-H1 mRNA or NDPK-A protein levels of primary or metastatic ET. NDPK-A protein levels were high and comparable to those of MCF-7 breast-cancer cells and to aggressive stage-IV neuroblastoma cell lines. nm23-H2/NDPK-B expression in ET was slightly more variable but generally lower than in MCF-7 cells. In the immunohistochemical analysis, however, discrepancies in the reactivity patterns with...Continue Reading

References

Mar 1, 1992·The Journal of Clinical Investigation·D KeimS M Hanash
Feb 20, 1991·Journal of the National Cancer Institute·C HennessyT W Lennard
Apr 6, 1988·Journal of the National Cancer Institute·P S SteegM E Sobel
Jan 1, 1993·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·A AyhanE Tahara

❮ Previous
Next ❯

Citations

Jul 6, 2000·Archives of Medical Research·D Utrera-BarillasL Benítez-Bribiesca
Oct 11, 1995·Nucleic Acids Research·M HildebrandtM Véron
Mar 1, 1997·Journal of Cutaneous Pathology·J KanitakisA Claudy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.