High-oleate yeast oil without polyunsaturated fatty acids

Biotechnology for Biofuels
Vasiliki TsakraklidesElena E Brevnova

Abstract

Oleate-enriched triacylglycerides are well-suited for lubricant applications that require high oxidative stability. Fatty acid carbon chain length and degree of desaturation are key determinants of triacylglyceride properties and the ability to manipulate fatty acid composition in living organisms is critical to developing a source of bio-based oil tailored to meet specific application requirements. We sought to engineer the oleaginous yeast Yarrowia lipolytica for production of high-oleate triacylglyceride oil. We studied the effect of deletions and overexpressions in the fatty acid and triacylglyceride synthesis pathways to identify modifications that increase oleate levels. Oleic acid accumulation in triacylglycerides was promoted by exchanging the native ∆9 fatty acid desaturase and glycerol-3-phosphate acyltransferase with heterologous enzymes, as well as deletion of the Δ12 fatty acid desaturase and expression of a fatty acid elongase. By combining these engineering steps, we eliminated polyunsaturated fatty acids and created a Y. lipolytica strain that accumulates triglycerides with > 90% oleate content. High-oleate content and lack of polyunsaturates distinguish this triacylglyceride oil from plant and algal derived oil...Continue Reading

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Citations

May 31, 2019·Microbial Biotechnology·Macarena LarroudeTristan Rossignol
Jun 14, 2020·FEMS Yeast Research·Annapurna KamineniVasiliki Tsakraklides
Jan 25, 2020·Biotechnology Letters·Macarena LarroudeTristan Rossignol
Jan 9, 2021·Biotechnology for Biofuels·Hannah G BlitzblauA Joe Shaw
Mar 21, 2021·Biotechnology for Biofuels·Yanbin LiuLianghui Ji
Jun 11, 2021·Applied Microbiology and Biotechnology·Daniel Ruben Akiola SanyaMahesh B Khot

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Datasets Mentioned

BETA
AEJ60084.1
CAA51674.1
CAA02067.1
EGU12399.1
BAH85840.1

Methods Mentioned

BETA
genetic modifications
PCR

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