PMID: 1495924Jan 1, 1992

High osmotic pressure enables fine ultrastructural and cytochemical studies on Pneumocystis carinii. I. Epon embedding

Parasitology Research
F PalluaultD Camus

Abstract

High osmotic pressure was used to preserve the ultrastructure of rabbit-, SCID mouse-, and rat-derived Pneumocystis carinii organisms from osmotic stress during fixation. Organelles and cytosol were well preserved within the tonicity range of 850-1,300 mosmol. Under these experimental conditions, we determined that the endoplasmic reticulum was well developed in all parasite stages and could observe the Golgi complex, autophagic vacuoles, dense bodies, type II endoplasmic saccules, and the recently described outer surface membrane, which was found in all parasite stages. The biological implications of these findings are discussed.

References

May 1, 1992·European Journal of Epidemiology·E Dei-CasD Camus
Jan 1, 1993·Biology of the Cell·E M AliouatD Camus
Sep 1, 1996·The Journal of Eukaryotic Microbiology·C CreusyE Dei-Cas
Jun 23, 2011·PloS One·Anna MartinezCécile-Marie Aliouat-Denis
Sep 10, 2009·The Journal of Eukaryotic Microbiology·Anna MartinezCécile-Marie Aliouat-Denis
Jul 1, 1996·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·J C CailliezE Dei-Cas

Citations

Apr 20, 1978·Zeitschrift Für Parasitenkunde·M E VossenA M Stadhouders
Jan 1, 1990·Biology of the Cell·F PalluaultD Camus
Mar 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M H Nielsen, O P Settnes
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D M SchmatzM J Turner
Sep 1, 1990·The Journal of protozoology·J A De StefanoP D Walzer
Jan 1, 1989·The Journal of protozoology·Y Yoshida
Jan 1, 1989·The Journal of protozoology·J J RuffoloP D Walzer
Aug 1, 1989·The Journal of Infectious Diseases·B SoulezD Camus
Apr 1, 1987·American Journal of Clinical Pathology·A ul HaqueM N Hart
Jan 1, 1985·The Journal of Infectious Diseases·N G HenshawA M Collier
Aug 1, 1970·The Journal of protozoology·J Vavra, K Kucera
Jan 1, 1991·Parasitology Today·F PalluaultD Camus

Related Concepts

Epon 864 of EPON
Golgi Apparatus
Pneumocystis Infections
Sample Fixation
Plastic Embedding
Cell Nucleus
Embedding
Chinchilla Rabbits
Pneumocystis jiroveci
Biologic Preservation

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