High oxygen environment during pregnancy rescues sickle cell anemia mice from prenatal death

Blood Cells, Molecules & Diseases
Lin YeYuet Wai Kan

Abstract

Several mouse models of sickle cell disease have been developed for the study of the pathophysiology of sickle cell disease and the investigation of drug and gene therapies. In previous years, we produced a sickle cell anemia mouse model in which the endogenous mouse alpha- and beta-globin genes were knocked out and replaced by the human alpha- and beta(s)-globin transgenes. The beta(s)-globin gene was contained in a 240 kb YAC that preserved the entire native genomic context of the beta-globin locus. These mice have hemolytic anemia, reticulocytosis and irreversible sickle cells in the peripheral blood, as well as other pathological features of sickle cell disease. However, in the embryo, the gamma-globin, like the mouse embryonic globin, declined quickly, and was replaced by beta(s)-globin expression from 12 days of gestation. The low level of fetal hemoglobin expression in utero led to intrauterine sickling and fetal death so that very few live-born sickle cell anemia mice could be obtained. To rescue these mice from intrauterine death, we investigated the effect of placing the pregnant mothers in a high O(2) environment. From the tenth day of gestation onwards, we placed the mothers into a chamber containing 50% O(2) and ke...Continue Reading

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M E FabryF Costantini
Feb 1, 1986·Obstetrics and Gynecology·D R PowarsP R Manning
Apr 30, 1973·JAMA : the Journal of the American Medical Association·A P Gelpi, R P Perrine
Feb 4, 1982·The New England Journal of Medicine·S H EmburyW C Mentzer
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K M GaenslerY W Kan
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K R PetersonG Stamatoyannopoulos
Nov 5, 1997·Science·T M RyanT M Townes
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·J C ChangK M Gaensler
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Judy C ChangYuet Wai Kan
Jun 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Swee Lay TheinMark Lathrop

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Citations

Dec 15, 2010·Cell Host & Microbe·Gleb PishchanyEric P Skaar
Sep 25, 2017·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Bradley H RosenJohn F Engelhardt

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