High oxygen tension increases itaconic acid accumulation, glucose consumption, and the expression and activity of alternative oxidase in Aspergillus terreus

Applied Microbiology and Biotechnology
Ákos P MolnárLevente Karaffa

Abstract

Itaconic acid is a five-carbon dicarboxylic acid with an unsaturated alkene bond, frequently used as a building block for the industrial production of a variety of synthetic polymers. It is also one of the major products of fungal "overflow metabolism" which can be produced in submerged fermentations of the filamentous fungus Aspergillus terreus. At the present, molar yields of itaconate are lower than those obtained in citric acid production in Aspergillus niger. Here, we have studied the possibility that the yield may be limited by the oxygen supply during fermentation and hence tested the effect of the dissolved oxygen concentration on the itaconic acid formation rate and yield in lab-scale bioreactors. The data show that a dissolved oxygen concentration of 2% saturation was sufficient for maximal biomass formation. Raising it to 30% saturation had no effect on biomass formation or the growth rate, but the itaconate yield augmented substantially from 0.53 to 0.85 mol itaconate/mol glucose. Furthermore, the volumetric and specific rates of itaconic acid formation ameliorated by as much as 150% concurrent with faster glucose consumption, shortening the fermentation time by 48 h. Further increasing the dissolved oxygen concentr...Continue Reading

References

Dec 10, 2002·Archives of Microbiology·Erzsébet FeketeChristian P Kubicek
Apr 17, 2003·Applied Microbiology and Biotechnology·Levente Karaffa, Christian P Kubicek
Mar 17, 2004·Applied Microbiology and Biotechnology·K Yabe, H Nakajima
Jul 1, 2004·Critical Reviews in Biotechnology·Zhonghu BaiBrian McNeil
Apr 25, 2007·Bioresource Technology·Lies DwiartiMitsuyasu Okabe
Aug 19, 2007·Journal of Bioscience and Bioengineering·Long-Shan T LaiChi-Chu Lo
Dec 1, 1983·Biotechnology and Bioengineering·R ManfrediniG Donati
Dec 2, 2008·Biotechnology Advances·Felix Garcia-Ochoa, Emilio Gomez
Apr 16, 2010·Annals of the New York Academy of Sciences·Brahmchetna BediRoberto Pacifici
Jul 4, 2012·Applied Microbiology and Biotechnology·Anja KuenzKlaus-Dieter Vorlop
Feb 20, 2013·Frontiers in Microbiology·Matthias G SteigerMichael Sauer
Sep 26, 2013·Biochemical Society Transactions·Luke YoungAnthony L Moore
Jan 21, 2014·Microbial Cell Factories·Laura van der StraatLeo H de Graaff
Jul 30, 2016·Microbial Cell Factories·Abeer H HossainPeter J Punt
Nov 11, 2016·Plant Signaling & Behavior·Bedabrata SahaSanjib Kumar Panda
Jan 7, 2017·PloS One·Wilson Garcia-NetoLuis Alberto Luévano-Martínez
Apr 21, 2017·Critical Reviews in Biotechnology·Richa Bafana, R A Pandey
Apr 26, 2017·Biotechnology Progress·Badal C SahaMichael J Bowman
Jan 5, 2018·Biotechnology Letters·Meilin ZhaoBin Zhuge
Feb 9, 2018·Mitochondrion·Allison E McDonald, Dmytro V Gospodaryov
Feb 28, 2018·3 Biotech·Juliana Cunha da CruzEliana Flávia Camporese Sérvulo
Mar 15, 2018·Applied Microbiology and Biotechnology·Anja Kuenz, Susan Krull
Apr 19, 2018·Trends in Plant Science·Jennifer SelinskiJames Whelan

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Citations

Feb 14, 2019·Applied Microbiology and Biotechnology·Levente Karaffa, Christian P Kubicek
Jun 20, 2019·Polymers·Bernadette-Emőke Teleky, Dan Cristian Vodnar
Oct 17, 2019·Biotechnology Letters·Nándor NemestóthyKatalin Bélafi-Bakó
Mar 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Amparo Jiménez-QueroVincent Phalip
Jul 25, 2019·Frontiers in Microbiology·István S KolláthLevente Karaffa
Jul 3, 2021·Microorganisms·Levente KaraffaChristian P Kubicek
Oct 13, 2021·ACS Synthetic Biology·Andrew D McNaughtonNeeraj Kumar

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BETA
AAJN01000000

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BETA
PCR

Software Mentioned

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