High-performance affinity chromatography of basic fibroblast growth factor on polystyrene sulphonate resins modified with serine

Journal of Chromatography
M A Jacquot-DourgesJ Jozefonvicz

Abstract

Basic fibroblast growth factor (bFGF) is a heparin-binding growth factor, so it was usually purified by affinity chromatography on a heparin-grafted support. It was previously observed that, among several modified polystyrene that exhibit anticoagulant heparin-like properties, insoluble polystyrenes modified by chlorosulphonation substituted with serine (PSSer) develop specific interactions with bFGF in solution. These PSSer resins were used as stationary phases in high-performance affinity chromatography in order to separate the radiolabelled growth factor from a bovine brain crude extract. The growth factor is strongly bound to the solid phase, as demonstrated by the adsorption isotherms. It can be adsorbed on the resin at low ionic strength and be desorbed by raising the salt concentration in the eluent. The effects of flow-rate, of the initial buffer and of the slope of the salt gradient on the adsorption and on the desorption of bFGF were investigated in order to study the thermodynamic and the kinetic parameters of the interactions and to define the optimum conditions for a fast and efficient separation of the growth factor.

References


❮ Previous
Next ❯

Citations

Aug 15, 1994·Journal of Biotechnology·N Labrou, Y D Clonis
Feb 22, 1991·Journal of Chromatography·M A Jacquot-DourgesJ Jozefonvicz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.