High-performance bioelectronic tongue using ligand binding domain T1R1 VFT for umami taste detection

Biosensors & Bioelectronics
Sae Ryun AhnTai Hyun Park

Abstract

Numerous efforts have been made to measure tastes for various purposes. However, most taste information is still obtained by human sensory evaluation. It is difficult to quantify a degree of taste or establish taste standard. Although artificial taste sensors called electronic tongues utilizing synthetic materials such as polymers, semiconductors, or lipid membranes have been developed, they have limited performance due to their low sensitivity and specificity. Recently, bioelectronic tongues fabricated by integrating human taste receptors and nanomaterial-based sensor platforms have been found to have high performance for measuring tastes with human-like taste perception. However, human umami taste receptor is heterodimeric class C GPCR composed of human taste receptor type 1 member 1 (T1R1) and member 3 (T1R3). Such complicated structure makes it difficult to fabricate bioelectronic tongue. The objective of this study was to develop a protein-based bioelectronic tongue for detecting and discriminating umami taste with human-like performance using umami ligand binding domain called venus flytrap (VFT) domain originating from T1R1 instead of using the whole heterodimeric complex of receptors. Such T1R1 VFT was produced from Esc...Continue Reading

Citations

Dec 4, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiao WuKiyoshi Toko
Jan 25, 2020·Science China. Life Sciences·Dongseok MoonTai Hyun Park
Mar 6, 2019·Sensors·Marcin ZabadajPatrycja Ciosek-Skibińska
Jun 18, 2020·Science Advances·Jeonghee YeomHyunhyub Ko
Jan 7, 2022·ACS Applied Materials & Interfaces·Jin-Young JeongSeunghun Hong

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