High-performance capillary electrophoretic characterization of different types of oligo- and polysialic acid chains

Analytical Biochemistry
M C ChengY Inoue

Abstract

We have carried out comparative structural analysis of novel oligo- and polysialic acid chains from diverse sources. Controlled acid hydrolysates of (a) colominic acid, alpha2-->8-linked homopolymer of N-acetylneuraminic acid (Neu5Ac), (b) alpha2-->8-linked oligo/polyNeu5Gc chains present in rainbow trout egg polysialoglycoprotein, and (c) alpha2-->8-linked oligomers of deaminoneuraminic acid (KDN) residues of KDN-rich glycoprotein derived from rainbow trout vitelline envelope were analyzed by high-performance capillary electrophoresis (HPCE). The results showed that three different types of alpha2-->8-linked oligosialic acids having same degree of polymerization can be separated by HPCE. A partial hydrolysate of colominic acid with mild acid was shown by CE to form intramolecular esters during the controlled hydrolysis and the subsequent workup procedure. In contrast, lactonization of (-->5-Oglycolyl-Neu5Gcalpha2-->)n, alpha2-->5-Oglycolyl-linked homopolymer of N-glycolylneuraminic acid (Neu5Gc) present in the egg jelly coat of sea urchin, did not take place as readily as in (-->8Neu5Acalpha2-->)n.

References

Aug 14, 1978·Biochemical and Biophysical Research Communications·S Inoue, M Iwasaki
Feb 1, 1992·Glycobiology·A Varki
Feb 1, 1992·Glycobiology·F A Troy
Mar 13, 1980·Biochemical and Biophysical Research Communications·S Inoue, M Iwasaki
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·S Kitazume-KawaguchiW J Lennarz

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Citations

Jun 4, 2008·Applied Microbiology and Biotechnology·Yanhong LiXi Chen
Sep 20, 2012·The Journal of Biological Chemistry·Fang MaPascal Gagneux
Sep 14, 2001·Glycobiology·S InoueY Inoue
Dec 19, 2009·Chemistry and Physics of Lipids·Teresa JanasTadeusz Janas
Mar 31, 2004·Analytical Biochemistry·Vincent E TurulaChul Yoo
Jul 25, 2000·Analytical Biochemistry·M Koketsu, R J Linhardt
Feb 26, 1999·Analytical Biochemistry·M C ChengS H Wu
Jul 21, 2001·The Journal of Organic Chemistry·Y P YuS H Wu
Aug 21, 2007·Analytical Chemistry·Sebastian P GaluskaHildegard Geyer

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