High-performance liquid chromatographic analysis of ampicillin.

Journal of Pharmaceutical Sciences
K Tsuji, J H Robertson

Abstract

A high-pressure liquid chromatographic method for the analysis of ampicillin is described. The method uses a 1-m long stainless steel column packed with anionic exchange resin, with a mobile phase of 0.02 M NaNO3 in 0.01 M pH 9.15 borate buffer at a flow rate of 0.45 ml/min. The degradation products of ampicillin, penicillenic and penicilloic acids of ampicillin, can be separated and quantitated in less than 12 min of chromatographic time. The relative standard deviation for the analysis of ampicillin is less than 1%, and the method is sensitive to approximately 20 ng of ampicillin/sample injected. The method was applied to the analysis of various pharmaceutical preparations of ampicillin. It is also applicable, with a slight modification, for the analysis of penicillins G and V.

References

Apr 1, 1967·The Analyst·J W SmithV J Patel

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Citations

Nov 15, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·S Kitazawa, T Komuro
Mar 1, 1996·Journal of Pharmaceutical and Biomedical Analysis·Y ZhuJ Hoogmartens
Mar 11, 1979·Journal of Chromatography·R B HagelD Humphrey

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