PMID: 2100624Jan 1, 1990Paper

High-performance liquid chromatographic analysis of the new antitumour drug SK&F 104864-A (NSC 609699) in plasma

Journal of Pharmaceutical and Biomedical Analysis
Jos H BeijnenW J Underberg

Abstract

A high-performance liquid chromatographic (HPLC) assay is described for the determination of the new, investigational antitumour drug SK&F 104864-A and its lactone ring opened form (SK&F 105992). The analytical methodology reported here involves a protein precipitation step with methanol as sample pretreatment procedure. The instability of the drug necessitates that the plasma fraction is obtained within 5 min after blood sampling by centrifugation, immediately followed by protein precipitation with cold methanol (-30 degrees C). The methanolic extract can be stored at -30 degrees C for several days without deterioration of the analyses. Stability data of the drug and its lactone ring opened metabolite in plasma and after methanolic extraction are discussed. The parent drug and the metabolite are separated by reversed-phase ion-pair liquid chromatography on a LiChrosorb RP-18 column, using methanol-water eluent (pH 6.0) with sodium dioctylsulphosuccinate (DOSS) as ion-pairing agent and fluorescence detection. The proposed method has been validated and, subsequently, implemented in a phase I clinical trial for pharmacokinetic evaluation of the new cytotoxic agent.

References

Jan 1, 1990·Journal of Pharmaceutical and Biomedical Analysis·W J UnderbergJ H Beijnen

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Citations

Jan 1, 1996·Investigational New Drugs·C F StewartC B Pratt
Jan 1, 1994·Cancer Chemotherapy and Pharmacology·P J O'DwyerK S Yao
Jan 1, 1995·Cancer Chemotherapy and Pharmacology·L J van WarmerdamJ H Beijnen
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