High-performance liquid chromatographic analysis of glutathione and its thiol and disulfide degradation products

Journal of Chromatography
A F SteinC D Klaassen

Abstract

A rapid and sensitive high-performance liquid chromatographic method for quantitation of picomole levels of glutathione, glutathione disulfide, cysteine, cystine, cysteinylglycine, cysteinylglycine disulfide and cysteine glutathione-mixed disulfide in biological samples is described. The compounds were separated isocratically on a reversed-phase column by ion-pair chromatography. The mobile phase consisted of an aqueous buffer containing 0.1 M monochloroacetic acid and 3.3 mM 1-heptanesulfonic acid (pH 2.60)-methanol-N,N-dimethylformamide (96.5:3.0:0.5). After chromatographic separation, the disulfides were reduced by a potential (-1.0 V) from a battery, with subsequent detection of all thiols by electrochemical oxidation (+0.15 V) with a dual gold-mercury electrode. Thiol and disulfide concentrations were determined in tissue extracts (liver and kidney) and fluids (bile and plasma) from control rats and rats treated with acivicin, an inhibitor of gamma-glutamyltranspeptidase. A marked increase in biliary glutathione concentration was observed in treated animals with a corresponding decrease in cysteine and cysteinylglycine concentrations. The results demonstrate that this method is useful for measuring glutathione and its degr...Continue Reading

References

Aug 22, 1977·Biochemical and Biophysical Research Communications·D J Reed, S Orrenius
Oct 1, 1976·Analytical Biochemistry·J BulterJ D Schulman
Jan 1, 1985·Annual Review of Pharmacology and Toxicology·N KaplowitzM Ookhtens
Feb 1, 1985·Analytical Biochemistry·A J Alpert, H F Gilbert

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Citations

Feb 1, 1989·Pflügers Archiv : European journal of physiology·M SuzukiT Miyahara
Jun 17, 1994·Journal of Chromatography. B, Biomedical Applications·A Rodriguez-ArizaJ López-Barea
Jan 1, 1992·Journal of Pharmaceutical and Biomedical Analysis·M C Gennaro, C Abrigo
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M D AleoP J Kostyniak
Jan 1, 1990·Experimental Pathology·M Kretzschmar, W Klinger
Oct 29, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·M W DaveyM Van Montagu
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Miranda VerschraagenWim J F van der Vijgh
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·A RompelM P Klein
May 1, 1988·Toxicology and Applied Pharmacology·A F SteinC D Klaassen
Oct 10, 2001·Therapeutic Drug Monitoring·D StempakS Baruchel
Apr 30, 2003·Journal of Biochemical and Molecular Toxicology·Lorelle I BerkeleyHerbert T Nagasawa
Jan 22, 1988·Journal of Chromatography·S Velury, S B Howell
Apr 19, 1991·Journal of Chromatography·K MertensA Vercruysse
Jul 17, 1998·Journal of the Neurological Sciences·M GuA H Schapira
Nov 15, 1988·Biochemical Pharmacology·Z GregusC D Klaassen
Dec 11, 1992·Toxicology and Applied Pharmacology·J W BaumanC D Klaassen
May 1, 1992·Toxicology and Applied Pharmacology·Z GregusC D Klaassen
Jul 1, 1990·Journal of Pharmaceutical Sciences·S M LunteP T Kissinger

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