PMID: 7019414Jun 1, 1981Paper

High-performance liquid chromatographic analysis of captopril in plasma

Journal of Pharmaceutical Sciences
B JarrottW J Louis

Abstract

A sensitive assay is described for quantitating plasma captopril levels. Captopril is an orally active inhibitor of angiotensin-converting enzyme. Blood from patients taking this drug was collected into tubes containing edetate and ascorbic acid, and the plasma was separated by centrifugation. After addition of an internal standard, the plasma was deproteinized and the supernate was adjusted to pH 6.5 N-(1-Pyrene)-maleimide was added to derivatize captopril and an internal standard to fluorescent adducts. These derivatives then were extracted into ethyl acetate-benzene (1:1) and separated from other derivatized thiols by high-performance liquid chromatography. The sensitivity of the assay was 150 pmoles/ml. Preliminary pharmacokinetics were obtained in patients taking captopril chronically for essential hypertension. After administration of 100 mg of captopril for patients who had fasted overnight, the plasma levels rose rapidly; peak levels were obtained at approximately 37 min. Thereafter, the plasma levels declined rapidly, and the terminal half-life was approximately 40 min. In these patients, the time course of the plasma levels did not reflect changes in blood pressure, so there appeared to be no direct relationship betwe...Continue Reading

References

Jun 29, 1976·Biochemistry·C W Wu, L R Yarbrough
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Citations

Jan 15, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·C Grönhagen-RiskaF Fyhrquist
Dec 1, 1981·Journal of Pharmacological Methods·D ArndtsH J Förster
May 10, 1985·Journal of Chromatography·M AhnoffJ Vessman
Jan 1, 1985·Journal of Pharmaceutical and Biomedical Analysis·D Perrett, S R Rudge
Jan 1, 1985·Journal of Pharmaceutical and Biomedical Analysis·J FarquharM Martin-Smith
Oct 1, 1993·Journal of Pharmaceutical and Biomedical Analysis·M I AlberoV Ródenas
Apr 1, 1995·Journal of Pharmaceutical and Biomedical Analysis·A JankowskiH Lamparczyk
Jan 24, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·C ArroyoJ Bonal
May 1, 1997·Journal of Pharmaceutical and Biomedical Analysis·M BahmaeiM Mahmoudian
Feb 1, 1992·British Journal of Clinical Pharmacology·J R WadeH L Elliott
Oct 1, 1989·Journal of Clinical Pharmacy and Therapeutics·G G Skellern
Dec 1, 1982·Journal of Clinical and Hospital Pharmacy·M J KendallM H Thomson
Apr 1, 1986·British Journal of Clinical Pharmacology·N HockingsJ L Reid
Mar 1, 1984·British Journal of Clinical Pharmacology·C RicherJ F Giudicelli
Jan 18, 2006·Journal of Pharmaceutical and Biomedical Analysis·Taomin HuangGengli Duan
Jan 1, 1986·Medicinal Research Reviews·O H Drummer, B Jarrott

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