PMID: 9187406Apr 25, 1997Paper

High-performance liquid chromatographic assay of 5-fluorouracil in human erythrocytes, plasma and whole blood

Journal of Chromatography. B, Biomedical Sciences and Applications
W WattanatornK E Kendle

Abstract

A HPLC assay method was modified and validated for the determination of 5-fluorouracil in human red blood cells, plasma and whole blood with a two-fold increased sensitivity (detection limit=10 ng/ml). The assay was linear from 25 to 1500 ng/ml and the accuracy ranged from 96.7 to 103.2% at 25 ng/ml, 94.8 to 99.4% at 500 ng/ml, and 98.9 to 99.5% at 1500 ng/ml. Intra-assay and inter-assay coefficients of variation were less than 8% over the range of concentrations and less than 8% over 10 days of analysis. After intravenous bolus and infusion of 5-fluorouracil in patients with colorectal cancer, the concentrations of 5-fluorouracil in whole blood were 108-111% of plasma concentrations, while packed red blood cells levels were 8-15% of plasma concentrations in the five patients studied. By utilising basic analytical hardware, this represents an accurate, precise, reproducible and affordable method for 5-fluorouracil pharmacokinetics investigation and therapeutic drug monitoring.

References

Oct 23, 1992·Journal of Chromatography·M Barberi-HeyobB Weber
Apr 1, 1989·Clinical Pharmacokinetics·R B Diasio, B E Harris
Jan 1, 1987·European Journal of Clinical Pharmacology·L J SchaafD G Perrier

❮ Previous
Next ❯

Citations

Apr 13, 2010·Analytical and Bioanalytical Chemistry·Massimo Breda, Simona Barattè
Apr 23, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hao-jie LuQing-yu Ou
Feb 12, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·W J LoosA Sparreboom
Jul 24, 2004·Critical Reviews in Clinical Laboratory Sciences·Herlinde DumezErnst A de Bruijn
Jul 29, 2003·Clinical Pharmacokinetics·Dirk Schrijvers
Feb 17, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·J EscoriazaJ Giráldez
Jul 30, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·L ZufíaJ Giráldez
Jun 22, 2005·Journal of Pharmaceutical and Biomedical Analysis·Rossella PisanoChristopher A James
Nov 29, 2005·Journal of Chemical Information and Modeling·Ivan SpasojevićGoran Bacić

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.