High-performance liquid chromatographic method with ultraviolet detection for the determination of dapsone and its hydroxylated metabolite in human plasma

Biomedical Chromatography : BMC
S Kwadijk, J Sastre Toraño

Abstract

A validated high-performance liquid chromatographic method with ultraviolet detection for the quantitative determination of dapsone (4,4'-diaminodifenyl sulfone, DDS) and a metabolite, hydroxylaminodapsone (4-amino-4-hydroxylaminodiphenyl sulfone, DDS-NOH), in human plasma is described. Human plasma was deproteinized with acetone and the clear supernatant solution after centrifugation was evaporated to dryness under a gentle stream of nitrogen at 70 degrees C. The residue was dissolved in a mixture of HPLC eluent and acetone (18:5 v/v) and an aliquot of this solution (50 microL) was injected onto the HPLC column. Dapsone, hydroxylaminodapsone and diazoxide as internal standard, were separated within 10 min by isocratic elution with water:acetonitrile:glacial acetic acid:triethylamine (80:20:1.0:0.5 by volume) as eluent. Detection was by ultraviolet at the wavelength of 295 nm. The within-day repeatability coefficients of variation were 3-5% for dapsone (0.301-20.0 mg/L, n = 5) and 3-5% for hydroxylaminodapsone (0.0948-6.32 mg/L, n = 5), whereas the between-day repeatability coefficients of variation were 3-8% (0.301-20.0 mg/L, n = 5) for dapsone and 4-10% for hydroxylaminodapsone (0.0948-6.32 mg/L, n = 5). The mean recoveries -...Continue Reading

References

Nov 1, 1995·Clinical Pharmacology and Therapeutics·A K MitraJ T Slattery

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Citations

Jan 28, 2014·PloS One·Taysa Ribeiro SchalcherMarta Chagas Monteiro
Jul 8, 2008·Life Sciences·Luis M VeggiAldo D Mottino
Sep 1, 2015·Acta Biomaterialia·Luis RojoJulio San Roman
Sep 14, 2010·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Jose Luiz Fernandes VieiraClaudio Guedes Salgado
Mar 24, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mateus M BergamaschiRegina Helena Costa Queiroz
Jul 18, 2017·Hormone Research in Pædiatrics·Rika KizuTomonobu Hasegawa
Jul 15, 2018·Journal of Separation Science·Sandia MachadoMarcela A Segundo
Nov 16, 2013·Therapeutic Drug Monitoring·Fernanda M L MouraJose L F Vieira
Mar 4, 2021·Journal of Pharmaceutical and Biomedical Analysis·Adrian Leistner, Ulrike Holzgrabe

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