High-performance liquid chromatographic method for analysis of 2',3'-dideoxyinosine in human body fluids

Journal of Chromatography
M E CarpenF Balis

Abstract

A paired-ion high-performance liquid chromatographic method was developed to measure concentrations of 2',3'-dideoxyinosine (ddI) in human plasma, urine and cerebrospinal fluid. Samples were prepared using a solid-phase extraction technique which allows for a five-fold concentration of the drug. 2'-Deoxyguanosine was added as an internal standard prior to the extraction. Recoveries for 2'-deoxyguanosine and ddI were 80 +/- 15 and 85 +/- 10%, respectively. Extracted samples were then injected onto a C18 column and eluted isocratically with a mobile phase containing 0.1% of the ion-pairing reagent, heptafluorobutyric acid, and 5% acetonitrile. The retention time was 7.4 min for 2'-deoxyguanosine and 8.4 min for ddI. The lower limit of detection for ddI is 0.1 microM. Using this technique the acid lability of ddI was demonstrated and the plasma concentration versus time profile from a patient receiving the drug was examined.

References

Dec 1, 1979·Computer Programs in Biomedicine·G D Knott
Feb 4, 1987·Nature·H Mitsuya, S Broder

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Citations

Oct 16, 1992·Pharmaceutisch Weekblad. Scientific Edition·R M FranssenJ H Beijnen
Jan 31, 2002·Journal of Chromatography. B, Biomedical Sciences and Applications·A S Pereira, R R Tidwell
Jun 12, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Naser L RezkAngela D M Kashuba
Jan 17, 1991·The New England Journal of Medicine·K M ButlerH Moss
Mar 1, 1992·British Journal of Clinical Pharmacology·D J BackR Macleod
Jun 1, 1995·Antimicrobial Agents and Chemotherapy·M E HawkinsF M Balis

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