High-performance liquid chromatographic method for the determination of etoposide in plasma using electrochemical detection

Journal of Chromatography
G F DuncanK A Pittman

Abstract

A quantitative analytical method has been established for the determination of a semi-synthetic epipodophyllotoxin, etoposide, in plasma. The method employs reversed-phase high-performance liquid chromatography and electrochemical detection. Sample preparation consisted of extraction with 1,2-dichloroethane followed by phase separation, evaporation of the organic phase, and reconstitution of the residue. Observed recoveries were 76.8 and 87.5% for 50 and 500 ng/ml, respectively. The method had a linear range of 10-1000 ng/ml. Correlation coefficients of 0.997 or greater were obtained during validation experiments and study sample analysis.

Citations

Jun 17, 1988·Pharmaceutisch Weekblad. Scientific Edition·J J Holthuis
Nov 17, 1993·Journal of Chromatography·E J Eisenberg, W M Eickhoff
Jan 31, 2002·Journal of Chromatography. B, Biomedical Sciences and Applications·T H Tsai
Jan 31, 2002·Journal of Chromatography. B, Biomedical Sciences and Applications·C L ChenF M Uckun
Aug 21, 2013·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Naseem AkhtarManu Jaggi
Oct 14, 2014·Journal of Thrombosis and Haemostasis : JTH·N van ReinF J M van der Meer

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