PMID: 11334350May 4, 2001Paper

High-performance liquid chromatographic-tandem mass spectrometric method for the determination of ethionamide in human plasma, bronchoalveolar lavage fluid and alveolar cells

Journal of Chromatography. B, Biomedical Sciences and Applications
John ConteElisabeth Zurlinden

Abstract

We have developed and validated an accurate, sensitive, and rapid high-performance liquid chromatographic-tandem mass spectrometric method (HPLC-MS-MS) for the determination of ethionamide in plasma, bronchoalveolar fluid (BAL) and alveolar cells (AC). The retention times for ethionamide, clemastine fumarate (internal standard for plasma), promethazine (internal standard for plasma) and propranolol (internal standard for BAL and AC) were approximately 2.62, 1.21, 2.14, and 2.22 min, respectively, with a total run time of 3.2 min. Ethionamide detection for plasma was carried out on a PE Sciex API III (Perkin-Elmer, Foster City, CA, USA). BAL and cell pellets and some plasma specimens were analyzed on a Micromass Quattro LC (Micromass Co., Manchester, UK). The detection limits for ethionamide were 0.05 microg/ml for plasma, and 0.005 microg/ml for BAL supernatants and alveolar cell suspensions.

Citations

Oct 29, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Weng Naidong
Apr 19, 2000·Antimicrobial Agents and Chemotherapy·J E ConteE Zurlinden
Jun 9, 2004·Analytical Biochemistry·Henri BenechJean-Robert Deverre
Jul 27, 2001·Journal of Mass Spectrometry : JMS
Apr 23, 2008·Journal of Separation Science·Yunsheng Hsieh
Jul 21, 2009·Experimental Physiology·Philip N Ainslie, Shigehiko Ogoh
Nov 3, 2010·The Journal of Physiology·Samuel J E LucasPhilip N Ainslie

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