PMID: 2110973Mar 1, 1990Paper

High-performance liquid chromatographic determination of the enantiomers of flecainide in human plasma and urine

Journal of Pharmaceutical Sciences
S Alessi-SeveriniS Gulamhusein

Abstract

A stereospecific high-performance liquid chromatographic method for the determination of (R,S)-flecainide acetate [(R,S)-N-(2-piperidylmethyl)-2,5-bis-(2,2,2-trifluoroethoxy)benzam ide acetate] in human plasma and urine is described. After addition of the internal standard [IS; (R,S)-N-(2-piperidylmethyl)-2,3-bis(2,2,2-trifluoroethoxy)- benzamide hydrochloride], a single-step extraction of alkalinized samples was performed with distilled diethyl ether. The organic layer was evaporated and the drug and IS were derivatized with 1-[(4-nitrophenyl)sulfonyl]-L-propyl chloride at 80 degrees C for 2 h. The diastereomeric derivatives of flecainide and IS were chromatographed on a C18 reversed-phase column with a mobile phase consisting of acetonitrile: water:triethylamine (45:55:0.2) at a flow rate of 1 mL/min. Flecainide diastereomers were separated with a resolution factor of 1.25 and detected by UV spectroscopy at a wavelength of 280 nm. An excellent linearity was observed between the peak area ratios (flecainide derivatives:IS) and plasma concentrations, and the intra- and interday coefficients of variation were always less than 9.8%. The lowest quantifiable concentration was set at 50 ng/mL for each enantiomer (CV of 4.9 and 4.4%)...Continue Reading

References

Mar 1, 1986·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·B Testa

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Citations

Nov 6, 1998·British Journal of Clinical Pharmacology·T J Campbell, K M Williams
May 16, 2003·Phytotherapy Research : PTR·Giovanni VaccaMauro A M Carai
Nov 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·T Breindahl

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