PMID: 3766956Aug 15, 1986Paper

High-performance liquid chromatographic separation of platinum complexes containing the cis-1,2-diaminocyclohexane carrier ligand

Analytical Biochemistry
S K MauldinS G Chaney

Abstract

Platinum drugs with the 1,2-diaminocyclohexane (dach) carrier ligand have shown great promise in cancer chemotherapy, but little is known about their metabolism in the body. Since it is possible to radiolabel the dach ligand, it should be possible to quantitate the biotransformation products of these drugs, provided a method were available to separate the biotransformation products. In this paper we describe a two-column high-performance liquid chromatography system which can be used to separate many likely dach-platinum biotransformation products from the parent compounds, and allow their identification. An initial separation on a reverse-phase Partisil ODS-3 column allowed resolution of the uncharged species. The peak fractions from this column were concentrated 10-fold and reinjected onto a cation exchange Partisil 10 SCX column to allow resolution of the positively charged species. This system allowed resolution of two prototype dach-platinum drugs, (cis-1,2-diaminocyclohexane)dichloroplatinum(II) and (cis-1,2-diaminocyclohexane)malonatoplatinum(II), the aquated species likely to form from these drugs, and the complexes formed when these compounds react with glutathione, metallothionein, and amino acids. By using cation exc...Continue Reading

References

Jul 1, 1982·Chemico-biological Interactions·P T Daley-Yates, D C McBrien
Dec 10, 1982·Science·S J Lippard

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Citations

Jan 1, 1994·Cancer Chemotherapy and Pharmacology·W P PetrosD L Trump
Jan 1, 1995·Cancer Chemotherapy and Pharmacology·D C ThompsonS G Chaney
Jan 1, 1990·Journal of Pharmaceutical and Biomedical Analysis·W A De WaalJ C Kraak
Jan 1, 1991·Journal of Pharmaceutical and Biomedical Analysis·S E NorthcottJ Dezwaan
Apr 29, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·F R LuoS G Chaney
Aug 23, 2005·Journal of Pharmacological and Toxicological Methods·A Lopez-FloresP Garcia-Lopez
Jan 1, 1995·Cancer Investigation·D C ThompsonS G Chaney
Mar 21, 2002·Cancer Investigation·Sridhar ManiStephen G Chaney
Mar 31, 1999·Journal of Biochemical and Molecular Toxicology·F R LuoS G Chaney

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