PMID: 8458899Mar 5, 1993Paper

High-performance liquid chromatographic measurement of leucine and alpha-ketoisocaproate in whole blood: application to fetal protein metabolism

Journal of Chromatography
J R Milley, J C Sweeley

Abstract

Evaluation of fetal protein metabolism requires measurement of a number of variables including umbilical blood flow, CO2 radioactivity, as well as plasma specific activities, whole blood concentration, and radioactivity of leucine and alpha-ketoisocaproate. This report details methods of analysis for whole blood concentration and radioactivity of leucine and alpha-ketoisocaproate using high-performance liquid chromatography that can be done on minimal blood volumes and are sufficiently accurate to detect the small arteriovenous differences important in measurements of fetal metabolism. Using these methods, the important components of fetal protein metabolism such as protein synthesis can be calculated with sufficient accuracy to detect differences as small as 10% provided appropriate experimental designs are used.

References

Dec 11, 1976·The Journal of Clinical Investigation·J A LemonsF C Battaglia
Jan 2, 1991·Journal of Chromatography·G L LoyP V Fennessey
Aug 1, 1989·The American Journal of Physiology·M E BlankD F Diedrich
Jan 1, 1987·Metabolism: Clinical and Experimental·L C van VeenF C Battaglia
Dec 7, 1984·Journal of Chromatography·B A BidlingmeyerT L Tarvin
Sep 1, 1981·Proceedings of the Society for Experimental Biology and Medicine·P MeierG Meschia

❮ Previous
Next ❯

Citations

Feb 20, 2008·Amino Acids·M FuchsJ Mühling
Jul 16, 2009·American Journal of Physiology. Endocrinology and Metabolism·Paul J RozanceLaura D Brown
Apr 1, 1994·The Journal of Clinical Investigation·J R Milley
Dec 17, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Marie J CzikkBryan S Richardson
Feb 1, 1997·The American Journal of Physiology·J R Milley
Aug 1, 1997·The American Journal of Physiology·J R Milley
May 12, 1998·The American Journal of Physiology·J R Milley
Jul 11, 2002·American Journal of Physiology. Cell Physiology·Laure VoisinSylvain Meloche
Oct 1, 1993·The American Journal of Physiology·J R Milley
Jul 1, 1996·The American Journal of Physiology·J R Milley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.