High-performance liquid chromatographic determination of thiopentone enantiomers in sheep plasma

Journal of Chromatography. B, Biomedical Applications
J L HuangC C Duke

Abstract

An HPLC method was developed to determine the plasma concentrations of R(+)- and S(-)-thiopentone for pharmacokinetic studies in sheep. The method required separation of the thiopentone enantiomers from the corresponding pentobarbitone enantiomers which are usually present as metabolites of thiopentone. Phenylbutazone was used as an internal standard. After acidification, the plasma sample were extracted with a mixture of ether and hexane (2:8). The solvent was evaporated to dryness and the residues were reconstituted with sodium hydroxide solution (pH 10). The samples were chromatographed on a 100 mm x 4 mm I.D. Chiral AGP-CSP column. The mobile phase was 4.5% 2-propanol in 0.1 M phosphate buffer (pH 6.2) with a flow-rate of 0.9 ml/min. This gave k' values of 1.92, 2.92, 5.71, 9.30 and 11.98 for R(+)-pentobarbitone, S(-)-pentobarbitone, R(+)-thiopentone, S(-)-thiopentone, and phenylbutazone, respectively. At detection wavelength of 287 nm, the limit of quantitation was 5 ng/ml for R(+)-thiopentone and 6 ng/ml for S(-)-thiopentone. The inter-day coefficients of variation at concentrations of 0.02, 0.1 and 8 micrograms/ml were, respectively, 4.8, 4.4 and 3.5% for R(+)-thiopentone and, respectively, 5.0, 4.3 and 3.9% for S(-)-thi...Continue Reading

References

Dec 1, 1985·Anaesthesia·H N ChanM D Boyd
Aug 1, 1983·Journal of Pharmaceutical Sciences·D R StanskiR K Richards
Aug 1, 1980·Anesthesiology·D R StanskiS M Kalman

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Citations

Mar 16, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·G CoppaA R Bonfigli
May 24, 2003·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Dennis J CordatoMichael K Morgan
Sep 28, 1999·British Journal of Pharmacology·D J CordatoG A Johnston
Apr 28, 2005·Neuroscience Letters·Laura LibrizziMarco de Curtis
Oct 3, 2006·Current Opinion in Anaesthesiology·L E Mather, S R Edwards

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