High-performance liquid chromatographic determination of fluconazole in plasma

Journal of Chromatography. B, Biomedical Applications
M CociglioF Bressolle

Abstract

A high-performance liquid chromatographic method with ultraviolet absorbance detection at 260 nm was developed for the analysis of fluconazole in plasma. The method involves sample clean-up by liquid-liquid extraction. The proposed technique is reproducible, selective, reliable and sensitive. Calibration standards were prepared in the range 1.25-20 mg/l. The limit of quantitation was 0.4 mg/l. The coefficients of variation were 5% between measurements of a single extract injected in duplicate, and 7% between two extractions of spiked samples at the same concentrations. The separation between fluconazole and endogenous substances was satisfactory. This method was designed in order to minimise the risk of interference from substances that could be co-administered to critically ill patients undergoing hemodiafiltration. With a run time below 5 min, the present method is rapid and easy to use for later clinical studies, as well as for routine monitoring.

References

Apr 1, 1988·Journal of Clinical Pharmacology·G FouldsD J Weidler
Apr 1, 1984·Forensic Science International : Synergy·H H HoppeP J Lincoln

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Citations

Jul 5, 2006·Biodegradation·Zhang ChaojieYu Hui
Feb 1, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·L Aït MoussaB Hüe
Apr 2, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Vikas KumarTimothy S Tracy
Jul 7, 2005·Biomedical Chromatography : BMC·Triporn Wattananat, Wiyada Akarawut
Feb 12, 2009·The Annals of Pharmacotherapy·Paul J Dentinger, Chad F Swenson
Dec 21, 2004·International Journal of Pharmaceutics·V PortaS Storpirtis
Apr 27, 1999·Therapeutic Drug Monitoring·L A MoraesG De Nucci
Jul 7, 2020·Journal of Pharmaceutical Health Care and Sciences·Jumpei SaitoAkimasa Yamatani

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