PMID: 9174278Apr 11, 1997Paper

High-performance liquid chromatographic determination of carbamazepine and metabolites in human hair

Journal of Chromatography. B, Biomedical Sciences and Applications
L A SarisP M Edelbroek

Abstract

To study the use of hair analysis in monitoring drug compliance and historical changes in pharmacokinetics we developed a method for the quantitative determination of the anti-epileptic drug carbamazepine (CBZ) and trans-10,11-dihydro-10,11-dihydroxy-carbamazepine (CBZ-diol) in hair from carbamazepine users. Digestion by 1 M NaOH was found to be the best method for isolating CBZ and CBZ-diol from hair, followed by solid-phase extraction and reversed-phase HPLC with UV detection. Recoveries from spiked hair samples were 76-86%. Within-day precision (C.V.; n = 10) for CBZ and CBZ-diol in hair of a CBZ user containing 10.9 microg/g CBZ and 3.2 microg/g CBZ-diol were 1.7 and 5.0%, respectively. Sectional hair analysis of a patient on a constant dosage of CBZ demonstrates an exponential decrease in hair concentrations of CBZ and CBZ-diol with increasing distance from the root, probably caused by shampooing. No CBZ-10,11-epoxide (CBZ-epox) could be detected. However, one component in the chromatogram is probably CBZ-beta-hydroxythioether, an adduct of CBZ-epox with cysteine, or acridinethioacetal, its rearrangement product. The concentration of this component does not decrease with increasing distance from the root.

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Citations

May 9, 2007·Analytical and Bioanalytical Chemistry·Frank Musshoff, Burkhard Madea
Mar 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniel F Chollet
May 16, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maria Augusta RaggiErnst Kenndler
Mar 18, 2006·Journal of Neurology, Neurosurgery, and Psychiatry·J WilliamsP E M Smith
Apr 21, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fritz Pragst, Marie A Balikova
Oct 30, 2016·Drug Testing and Analysis·Xin WangKristian Linnet
Feb 17, 2005·Electrophoresis·Vincenzo Pucci, Maria Augusta Raggi
Nov 26, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·Y Nakahara
Oct 27, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·R MandrioliM A Raggia

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