High performance liquid chromatographic determination of folic acid and its photodegradation products in the presence of riboflavin

Journal of Pharmaceutical and Biomedical Analysis
M J AkhtarI Ahmad

Abstract

A high performance liquid chromatographic procedure was developed to determine folic acid and its photodegradation products, p-aminobenzoic acid, pterine-6-carboxylic acid, p-aminobenzoyl-L-glutamic acid, and pteroic acid in the presence of riboflavin. The method involves reversed phase, paired-ion chromatography on mu-BondaPak C18 column using a UV detector (254 nm), and isocratic solvent system (at ambient temperature) comprising 0.017 M monobasic potassium phosphate, tetrabutyl ammonium hydroxide solution (20%, aqueous) and methanol (870:15:250, v/v). The range of quantitation for the individual compounds was found to be: p-aminobenzoic acid, 0.01 - 1.25 x 10(-5) M; pterine-6-carboxylic acid, 0.01-2.0 x 10(-5) M; p-aminobenzoyl-L-glutamic acid, 0.02-2.0 x 10(-5) M; pteroic acid, 0.02-2.5 x 10(-5) M; folic acid, 1.0-5.0 x 10(-5) M; riboflavin, 1.0-5.0 x 10(-5) M. Linear regression analysis of the data demonstrates adequate performance of the method in terms of accuracy and precision (R. S. D. 3%). The method is specific, rapid and convenient and has been applied to photodegradation studies of folic acid in the presence and absence of riboflavin.

References

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Citations

May 11, 2006·Analytical and Bioanalytical Chemistry·A Muñoz de la PeñaA Jiménez Girón
Aug 10, 2006·Analytical and Bioanalytical Chemistry·Kazutaka Hirakawa
Mar 31, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·J Rodríguez FloresM J Rodríguez Gómez
Nov 30, 2000·Journal of Pharmaceutical and Biomedical Analysis·M J AkhtarI Ahmad
Mar 5, 2003·Journal of Pharmaceutical and Biomedical Analysis·M Jamil AkhtarIqbal Ahmad
Mar 7, 2000·Journal of Pharmaceutical and Biomedical Analysis·M J AkhtarI Ahmad
Jan 4, 2007·The British Journal of Dermatology·M Der-PetrossianF Trautinger
Mar 2, 2012·Journal of the Science of Food and Agriculture·Taichang ZhangRimo Xi
Jan 23, 2010·Luminescence : the Journal of Biological and Chemical Luminescence·Jamshid L ManzooriJafar Soleymani
Aug 2, 2003·Phytochemical Analysis : PCA·Zhenghua Song, Lin Wang
Feb 20, 2007·Memórias do Instituto Oswaldo Cruz·Christine WhiteFred Longstaffe
May 1, 2016·Comprehensive Reviews in Food Science and Food Safety·Nicolas DelchierCatherine M G C Renard
Jun 29, 1999·Analytical Chemistry·R K Gilpin, L A Pachla

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