High performance liquid chromatography analysis of MESNA (2-mercaptoethane sulfonate) in biological samples using fluorescence detection

Biomedical Chromatography : BMC
Suneetha MareNuran Ercal

Abstract

MESNA is the sodium salt of 2-mercaptoethane sulfonate, a thiol-containing drug. It is an antioxidant used particularly in renal protection. Several studies have proved that MESNA has beneficial effects in ischemic acute renal failure where it scavenges reactive oxygen species (ROS), due to the presence of the thiol group. It also reduces the size of urinary bladder cancer. MESNA was proved to be effective in preventing hemorrhagic cystitis induced by high doses of several chemotherapeutic regimens such as cyclophosphamide and ifosfamide. It has been shown that MESNA functions as an uroprotective substance in drug-induced experimental bladder cancer models. Moreover, recent studies have suggested that it is also effective in reducing intestinal inflammation in colitis. Because of the increased level of interest in using MESNA for treating various disorders, a new and sensitive method was needed to understand the pharmacokinetics of this drug. Accordingly, we developed a new method for determining free MESNA in biological samples by using ThioGlo-3 [3H-Naphto [2,1-b] pyran, 9-acetoxy-2-(4-(2,5-dihydro-2, 5-dioxo-1H-pyrrol-1-yl) phenyl-3-oxo)] as the derivatizing agent. MESNA was detected fluorimetrically by reverse-phase HPLC us...Continue Reading

References

Jan 1, 1991·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·O R LeeuwenkampH M Pinedo
Sep 1, 1993·British Journal of Cancer·B Stofer-VogelB H Lauterburg
Oct 3, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M L HensleyD G Pfister
Mar 10, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·E MashiachB Kristal
May 4, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·N ErcalN Aykin
May 4, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·M VerschraagenW J van der Vijgh
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Miranda VerschraagenWim J F van der Vijgh
Jun 26, 2003·Digestive Diseases and Sciences·T ShustermanR Reshef
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN

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Citations

Mar 31, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun ChenDavid E Smith
Nov 17, 2015·Expert Review of Anticancer Therapy·Dahlia SalmanShereen Nabhani-Gebara
Sep 23, 2014·Journal of Chromatographic Science·Mohamed RizkYoumna M Abdallah
Jun 9, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Paulina FurmaniakRafał Głowacki
May 27, 2010·Chemical Society Reviews·Xiaoqiang ChenJuyoung Yoon
May 25, 2013·Chemical Society Reviews·Caixia YinSidian Li
Apr 10, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Toshimasa Toyo'oka

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