PMID: 11318417Apr 25, 2001Paper

High-performance liquid chromatography assay for simultaneous determination of dextromethorphan and its main metabolites in urine and in microsomal preparations

Journal of Chromatography. B, Biomedical Sciences and Applications
E K BendrissIrving W Wainer

Abstract

An HPLC method has been developed and validated for the determination of dextromethorphan, dextrorphan, 3-methoxymorphinan and 3-hydroxymorphinan in urine samples. Deconjugated compounds were extracted on silica cartridges using dichloromethane/hexane (95:05, v/v) as an eluent. Chromatographic separation was accomplished on a Phenyl analytical column serially connected with a Nitrile analytical column. The mobile phase consisted of a mixture of an aqueous solution, containing 1.5% acetic acid and 0.1% triethylamine, and acetonitrile (75:25, v/v). Compounds were monitored using a fluorescence detector. Calibration curves were linear over the range investigated (0.2-8.0 microM) with correlation coefficients >0.999. The method was reproducible and precise. Coefficients of variation and deviations from nominal values were both below 10%. For all the analytes, recoveries exceeded 77% and the limits of detection were 0.01 microM. The validated assay proved to be suitable for the determination of DEM metabolic indexes reported to reflect the enzymatic activity of the cytochrome P450s, CYP2D6 and CYP3A, both in vivo, when applied to urine samples from patients, and in vitro, when applied to samples from the incubation of liver microsom...Continue Reading

References

Sep 17, 1977·Lancet·A MahgoubR L Smith
May 1, 1986·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A KüpferG Pfaff

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Citations

Feb 12, 2011·Analytical and Bioanalytical Chemistry·Ruri Kikura-HanajiriYukihiro Goda
Feb 28, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lun-Yi ZangGuilin Qiao
Jan 29, 2013·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Dipti B Ruikar, Sadhana J Rajput
Jun 15, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ya-Juan LiuRu-Qin Yu
Jun 6, 2014·Pakistan Journal of Biological Sciences : PJBS·Sekar RajanK Elango
Apr 9, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Fahad I Al-JenoobiSaleh A Al-Suwayeh
Oct 9, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shyr-Yi LinMing-Thau Sheu
May 9, 2016·Analytica Chimica Acta·T Alvarez-SeguraM C García-Alvarez-Coque
Apr 18, 2014·Journal of Pharmacological Sciences·Ling-ling JiaXiao-dong Liu
Jan 7, 2015·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Fahad I Al-JenoobiSaleh A Al-Suwayeh
Feb 11, 2014·European Journal of Drug Metabolism and Pharmacokinetics·Fahad Ibrahim Al-JenoobiSaleh A Al-Suwayeh
May 11, 2018·European Journal of Drug Metabolism and Pharmacokinetics·Haitham AlRabiahFahad I Al-Jenoobi
Jul 11, 2018·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Ahmed A AlbassamFahad I Al-Jenoobi
Apr 9, 2021·Drug Metabolism and Personalized Therapy·Boon Hooi TanChin Eng Ong

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