High-performance liquid chromatography assay for moxifloxacin in brain tissue and plasma: validation in a pharmacokinetic study in a murine model of cerebral listeriosis.

Journal of Analytical Methods in Chemistry
Renaud RespaudMarie-Catherine Lott

Abstract

Moxifloxacin is a broad-spectrum antibacterial 8-methoxy-fluoroquinolone. In order to evaluate the pharmacokinetic properties of moxifloxacin in mouse plasma and brain tissue, we developed a high-performance liquid chromatography (HPLC) method. This study was based on single-drug delivery, intravenously dosed in a central listeriosis murine model. The method employed a reversed-phase Lichrospher RP-18 with a precolumn (250 × 4.6 mm) and a mobile phase composed of a mixture of acetonitrile, methanol, and citric buffer (pH = 3.5) with sodium dodecyl sulfate and tetrabutylammonium bromide. Fluorescence detection was performed at an excitation wavelength of 290 nm and an emission wavelength of 550 nm. The relative standard deviation of intra- and inter-day assays was <10%. This validated method led to a short retention time (8.0 min) for moxifloxacin. The standard curves were linear from 5-250 μg/L in plasma and from 0.1-2.5 μg/g of brain tissue. The limits of quantification were 5 μg/L in plasma and 0.1 μg/g in brain tissue. The method enabled the detection of systemic antimicrobial in plasma and in CNS in Listeria-infected mice. Injected moxifloxacin passed through the encephalic barrier within a 30 to 60 min after injection time...Continue Reading

References

Jan 1, 1996·Scandinavian Journal of Infectious Diseases·V Goulet, P Marchetti
Oct 1, 1996·Journal of Psychoactive Drugs·C Mallouh
Apr 8, 1999·Drugs·J A Balfour, L R Wiseman
Jun 26, 1999·The Journal of Antimicrobial Chemotherapy·H M SiefertH H Stass
Apr 25, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·B B BaM C Saux
Oct 16, 2001·Antimicrobial Agents and Chemotherapy·V Rodriguez-CerratoG H McCracken
Oct 27, 2001·Science·P GlaserP Cossart
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hairui LiangKevin M Sowinski
Jul 22, 2004·Expert Opinion on Pharmacotherapy·Herbert Hof
Jul 29, 2006·Journal of Pharmaceutical and Biomedical Analysis·Sevgi Tatar Ulu
Aug 8, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Aleksandra Laban-DjurdjevićPredrag Djurdjević
Feb 27, 2008·Antimicrobial Agents and Chemotherapy·S GrayoA Le Monnier
Mar 21, 2008·The Journal of Antimicrobial Chemotherapy·Kyriaki KanellakopoulouEvangelos J Giamarellos-Bourboulis
Jun 25, 2008·Antimicrobial Agents and Chemotherapy·Solène GrayoAlban Le Monnier
Mar 17, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A K Hemanth Kumar, Geetha Ramachandran
Aug 29, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J W C AlffenaarT S van der Werf

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Citations

Jun 4, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Joana SousaAmílcar Falcão
Jul 21, 2020·Critical Reviews in Analytical Chemistry·Akhila PyaramPinaki Sengupta
Mar 31, 2021·Biomedical Chromatography : BMC·Noha M El ZaharMichael G Bartlett

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Methods Mentioned

BETA
reversed-phase chromatography

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