High-performance liquid chromatography combined with intrinsic fluorescence detection to analyse melittin in individual honeybee (Apis mellifera) venom sac

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Jiangtao DongXiaoqing Miao

Abstract

Melittin is the major toxin peptide in bee venom, which has diverse biological effects. In the present study, melittin was separated by reverse-phase high-performance liquid chromatography, and was then detected using intrinsic fluorescence signal of tryptophan residue. The accuracy, linearity, limit of quantitation (LOQ), intra-day and inter-day precision of the method were carefully validated in this study. Results indicate that the intrinsic fluorescence signal of melittin has linear range from 0.04μg/mL to 20μg/mL with LOQ of 0.04μg/mL. The recovery range of spiked samples is between 81.93% and 105.25%. The precision results are expressed as relative standard deviation (RSD), which is in the range of 2.1-7.4% for intra-day precision and 6.2-10.8% for inter-day precision. Because of the large linear dynamic range and the high sensitivity, intrinsic fluorescence detection (IFD) can be used for analyzing melittin contents in individual venom sac of honeybee (Apis mellifera). The detected contents of melittin in individual bee venom sac are 0.18±0.25μg for one-day old honeybees (n=30), and 114.98±43.51μg for 25-day old (n=30) honeybees, respectively. Results indicate that there is large bee-to-bee difference in melittin content...Continue Reading

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Citations

Jun 12, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Filipa SobralIsabel C F R Ferreira
Jul 16, 2020·Toxins·Hesham El-SeediShaden Khalifa
Mar 3, 2018·Toxicon : Official Journal of the International Society on Toxinology·Suvimol SomwonginWantida Chaiyana
May 1, 2021·Molecular Immunology·Carlos Leónidas LeivaPablo Chacana
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Irina TanuwidjajaMirna Mrkonjić Fuka

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