High-performance liquid chromatography for the determination of three new fluoroquinolones, fleroxacin, temafloxacin and A-64730, in biological fluids

Journal of Chromatography
C KoechlinH Monteil

Abstract

High-performance liquid chromatographic procedures have been developed for the measurement of three new fluoroquinolones, fleroxacin, temafloxacin and A-64730, in serum, urine and bile. The sample treatment consists of a two-step chemical extraction. The three molecules are chromatographed on a C18 reversed-phase analytical column with spectrofluorimetric detection. At a signal-to-noise ratio of 4, the detection limits in serum are 2.5, 10 and 20 ng/ml, for fleroxacin, temafloxacin and A-64730, respectively. The calibration curves are rectilinear between these detection limits and 20 micrograms/ml. The intra- and inter-assay coefficients of variation are in the ranges 0.8-5.4 and 2.2-7.6%, respectively. These simple and reliable assay procedures will be of great interest for further pharmacokinetic studies and drug monitoring in hospital use.

Citations

Feb 3, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·N HobaraM Sakanashi
Jun 17, 2000·Journal of Pharmaceutical and Biomedical Analysis·V KapetanovićL J Ignjatović
Sep 14, 1999·The American Journal of Gastroenterology·C Y ChenX Z Lin
Oct 12, 1990·Journal of Chromatography·F JehlH Monteil

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