PMID: 9187389Apr 25, 1997Paper

High-performance liquid chromatography-ionspray mass spectrometry for the specific determination of digoxin and some related cardiac glycosides in human plasma

Journal of Chromatography. B, Biomedical Sciences and Applications
A TracquiP Mangin

Abstract

An original method based upon high-performance liquid chromatography coupled to ionspray mass spectrometry (HPLC-ISP-MS) has been developed for the identification and quantification in plasma of several cardiac glycosides, namely digoxin, digitoxin, lanatoside C and acetyldigitoxin. After single-step liquid-liquid extraction by chloroform-2-propanol (95:5, v/v) at pH 9.5 using oleandrin as an internal standard, solutes are separated on a 4 microm NovaPak C18 (Waters) column (150x2.0 mm, I.D.), using a gradient of acetonitrile-2 mM NH4COOH, pH 3 buffer (flow-rate 200 microl/min, post-column split 1:3). Detection is done by a Perkin-Elmer Sciex API-100 mass analyzer equipped with an ISP interface. In most instances the major ion observed is not [M+H]+ as expected, but [M+NH4]+. The mean retention times (min) are: lanatoside C, 5.74; digoxin, 6.00; digitoxin, 8.08, oleandrin, 8.30, acetyldigitoxin, 8.66 and 9.01 (isomers alpha and beta, respectively). The lower limits of detection in single ion monitoring mode range from 0.15 ng/ml (alpha- and beta-acetyldigitoxin) to 0.60 ng/ml (lanatoside C), making the method less sensitive than radioimmunoassay, whereas it is much more specific.

Citations

Nov 16, 2010·Analytical and Bioanalytical Chemistry·Sanae KannoOsamu Suzuki
May 21, 2005·International Journal of Legal Medicine·J PietschJ Dressler
Jun 15, 2007·International Journal of Legal Medicine·L FrommherzJ Beike
Mar 8, 2011·Forensic Science International : Synergy·Sarah KohlsJörg Teske
Aug 31, 2010·Journal of Pharmaceutical and Biomedical Analysis·Ha-Jeong KwonSeon-Pyo Hong
Jul 8, 2008·Forensic Science International : Synergy·Ibrahim A WasfiAnwar M Al Awadhi
Mar 5, 2008·Biomedical Chromatography : BMC·Brian J KirbyJashvant D Unadkat
Mar 15, 2016·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Diana N KnittelDietmar R Kammerer
Dec 27, 2011·Phytochemistry·Sarah KohlsJürgen Rullkötter
May 24, 2005·Journal of Pharmaceutical and Biomedical Analysis·Giorgio GrosaErika Del Grosso
Jul 22, 2011·International Journal of Analytical Chemistry·Paula MeloHelena M Teixeira
Feb 24, 2001·Journal of Hypertension·Y KomiyamaH Takahashi
Sep 15, 2000·Mass Spectrometry Reviews·J F Van BocxlaerA P De Leenheer
Jan 5, 2002·Journal of Chromatography. a·K ShimadaT Higashi
Nov 26, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·M J Bogusz
Jan 19, 2000·Journal of Agricultural and Food Chemistry·D M HolstegeF D Galey
May 26, 2005·Journal of Agricultural and Food Chemistry·Elizabeth R TorBirgit Puschner
Apr 15, 2004·Journal of Agricultural and Food Chemistry·Michael S FiligenziBirgit Puschner
Aug 22, 2000·Analytical Chemistry·X WangA Cisneros

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.