High-performance liquid chromatography-mass spectrometry-based acetylcholinesterase assay for the screening of inhibitors in natural extracts

Journal of Chromatography. a
Camiel F de JongH Irth

Abstract

The present paper describes a High-performance liquid chromatography-mass spectrometry (LC-MS) methodology for the screening of acetylcholinesterase (AChE) inhibitors in natural extracts. AChE activity of sample components is monitored by a post-column biochemical assay that is based on the separate, sequential mixing of AChE and acetylcholine, respectively, with the HPLC eluate. AChE inhibitors are detected by measuring a decrease of product formation using electrospray MS. Ammonium bicarbonate was used as buffer in order to achieve optimum compatibility between biochemical assay and MS detection conditions. The assay is robust and stable for over 13 h and compares favourably with other AChE assays in terms of stability and sensitivity. IC(50) values of 9-aminoacridine, galanthamine, gallamine, (-)-huperzine A and thioflavin T were determined to be 0.12, 0.38, 6.4, 0.46 and 3.2 microM, respectively. The assay was used to effectively identify an AChE inhibitor present in a crude extract of Narcissus c.v. "Bridal Crown".

Citations

Oct 11, 2007·Analytical and Bioanalytical Chemistry·Thomas Letzel
Nov 26, 2010·Analytical and Bioanalytical Chemistry·Jeroen KoolWilfried M A Niessen
Jul 20, 2011·Analytical and Bioanalytical Chemistry·Johanna GrassmannThomas Letzel
Feb 4, 2012·Analytical and Bioanalytical Chemistry·Tomáš Křížek, Anna Kubíčková
Jul 27, 2007·Natural Product Reports·Zhong Jin
Apr 11, 2012·International Journal of Molecular Sciences·Christiaan J MalherbeElizabeth Joubert
Jan 3, 2014·Biochemistry Research International·Vikas DhullVikas Hooda
Mar 19, 2014·Journal of Pharmaceutical and Biomedical Analysis·Wei LiuChanghong Wang
Sep 28, 2012·Expert Opinion on Drug Discovery·Ondrej HolasKamil Kuca
Feb 9, 2011·Journal of Pharmaceutical and Biomedical Analysis·Lygia Azevedo MarquesMartin Giera
Mar 18, 2016·Journal of Pharmaceutical and Biomedical Analysis·Christine M KaufmannThomas Letzel
Dec 15, 2010·Biomedical Chromatography : BMC·Lygia Azevedo MarquesWilfried Ma Niessen
Mar 24, 2016·Rapid Communications in Mass Spectrometry : RCM·Michael KrappmannThomas Letzel
Oct 22, 2013·Toxicon : Official Journal of the International Society on Toxinology·Reka A OtvosJeroen Kool
Jun 9, 2012·Journal of Pharmaceutical and Biomedical Analysis·Anna ZiemianinCatherine M Lange
Apr 16, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qiang FuShuying Liu
Jan 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingqian SuQi Chen
Feb 21, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Li De-QiangLi Shao-Ping
Aug 25, 2015·Chembiochem : a European Journal of Chemical Biology·Therese BurkhardtJohanna Grassmann
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Sep 17, 2009·Electrophoresis·Aiping Schuchert-Shi, Peter C Hauser
May 28, 2009·Journal of Separation Science·Yolanda Martín-BioscaMaria J Medina-Hernández
Aug 18, 2009·Analytical Chemistry·Eu Vian Tan, Christopher R Lowe
Jan 2, 2009·Journal of Natural Products·Agata PtakDominique Laurain-Mattar
May 22, 2007·Analytical Chemistry·R K Gilpin, C S Gilpin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.