High-performance liquid chromatography methods for the separation and quantitation of spironolactone and its degradation products in aqueous formulations and of its metabolites in rat serum

Journal of Chromatography. a
A M KaukonenJ P Mannermaa

Abstract

HPLC assays have been developed for the determination of spironolactone and its degradation products 7 alpha-thiospirolactone and canrenone in aqueous solutions of beta-cyclodextrins and for the determination of spironolactone and its metabolites 7 alpha-thiospirolactone, 7 alpha-thiomethylspirolactone, 6 beta-hydroxy-7 alpha-thiomethylspirolactone and canrenone in rat serum samples. Both methods were well suited for their respective applications, i.e., studying the stability of spironolactone in liquid formulations of beta-cyclodextrins and the oral absorption of spironolactone in rats. The HPLC method developed for spironolactone and its metabolites in rat serum requires very small volumes of serum making it possible to take several blood samples over a period of time.

References

Sep 1, 1988·The Journal of Pediatrics·S A AtkinsonB T Steele
Jun 1, 1996·Journal of Pharmaceutical and Biomedical Analysis·A JankowskiH Lamparczyk

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Citations

Feb 1, 2003·Journal of Pharmaceutical and Biomedical Analysis·Ji Y ZhangAlan P Breau
Dec 13, 2000·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·L BønløkkeH Lennernäs
Mar 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ji Y ZhangAlan P Breau
Dec 29, 2011·Journal of Biomedicine & Biotechnology·Da-Ming HuangYa-Juan Wang
Jun 3, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Sándor Görög
Nov 13, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Irena BaranowskaJacek Baranowski
Mar 3, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·J M SandallJ C McElnay
Apr 16, 2005·Drug Development and Industrial Pharmacy·P LangguthH Spahn-Langguth
Apr 28, 2005·Journal of Periodontal Research·M C B GrauballeP Holmstrup

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