High-performance liquid chromatography of sulphadimethoxine and its N1-glucuronide, N4-acetyl and N4-acetyl-N1-glucuronide metabolites in human plasma and urine

Journal of Chromatography
T B VreeM Shimoda

Abstract

Sulphadimethoxine is metabolized in humans by N1-glucuronidation and by N4-acetylation. Sulphadimethoxine-N1-glucuronide can be measured by the direct high-performance liquid chromatographic analysis and without enzymic deglucuronidation. The N1-glucuronide can be measured by an isocratic as well as by a gradient mobile phase. The group contribution of the N1-glucuronide moiety to the capacity factor is a reduction of 0.24 in the isocratic system and 0.55 in the gradient system. N4-Acetylation increases the capacity factor by a factor 1.4 in the isocratic system and by 1.06 in the gradient system.

Citations

Apr 27, 1990·Pharmaceutisch Weekblad. Scientific Edition·T B VreeM Shimoda
Oct 18, 1991·Pharmaceutisch Weekblad. Scientific Edition·T B VreeY A Hekster
Jan 1, 1992·Biopharmaceutics & Drug Disposition·T B VreeT Miura

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