PMID: 4096372Dec 1, 1985Paper

High-performance liquid chromatography of glucagon and related compounds

Analytical Biochemistry
G MazzolaG Carrea


Glycerolpropylsilane bonded phases have been found to adsorb peptides and proteins via ionic interactions. In this paper high-performance liquid chromatography separation of glucagon and related compounds, using a Diol-silica matrix, is described. Crystalline, commercial preparations of glucagon, when analyzed on LiChrosorb Diol columns eluted with low-ionic-strength acidic buffers, contained up to four contaminant peaks, in different numbers and ratios. Three of these contaminants, called A, C, and D, were recovered and characterized. Contaminant A, representing a few percent of the total, was a mixture of mono- and didesamidoglucagon, as shown by treatment with bis(I,I-trifluoroacetoxy)iodobenzene, with which it is possible to differentiate between carboxamide and carboxylic acid residues. Contaminant C, ranging from 0 to about 30% of the total, was N-terminal degraded glucagon. Contaminant D, ranging from a few percent to about 25% of the total, was (Met27 sulfoxide) glucagon.


Aug 1, 1979·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·W J Nooijen, H J Kempen
May 1, 1979·FEBS Letters·M D Bregman, V J Hruby
Jan 13, 1978·Biochemical and Biophysical Research Communications·M E Parham, G M Loudon
Jul 1, 1976·Journal of Chromatographic Science·F E Regnier, R Noel
Jan 1, 1983·Methods in Enzymology·F E Regnier
Oct 1, 1982·Analytical Biochemistry·W Kopaciewicz, F E Regnier
Jan 1, 1981·Archives of Biochemistry and Biophysics·B N Jones, R S Gurd

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Jul 20, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·I German, R T Kennedy

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