High-performance liquid chromatography separation and purification of cacao (Theobroma cacao L.) procyanidins according to degree of polymerization using a diol stationary phase

Journal of Agricultural and Food Chemistry
Mark A KelmHarold H Schmitz

Abstract

A new chromatographic approach for separating cacao procyanidins according to their degree of polymerization has been developed. It utilizes diol stationary phase columns operating in normal phase mode with a binary gradient of acidified acetonitrile and methanol-water. Performance of the diol stationary phase was evaluated on an analytical scale utilizing classical chromatographic conditions for the normal phase separation of procyanidins according to their degree of polymerization. The new separation approach was developed on an analytical scale but further extended to the preparative scale. These newly developed analytical and preparative high-performance liquid chromatography procedures were successfully applied to the separation, as well as isolation, of cacao procyanidins from unfermented cacao seeds. The degree of polymerization associated with each molecular weight fraction was determined by mass spectrometry.

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Citations

Dec 7, 2013·Dental Materials : Official Publication of the Academy of Dental Materials·Ana K Bedran-RussoAriene A Leme
Mar 12, 2011·Journal of Agricultural and Food Chemistry·Ryo FuruuchiMasao Hirayama
Mar 29, 2011·Journal of Agricultural and Food Chemistry·Brittany L WhiteRonald L Prior
Dec 20, 2011·Journal of Agricultural and Food Chemistry·Ana Rodriguez-MateosJeremy P E Spencer
Mar 8, 2012·Journal of Agricultural and Food Chemistry·Hongyu WangDejian Huang
Dec 19, 2012·Journal of Agricultural and Food Chemistry·Ying Yng ChoyAndrew L Waterhouse
May 24, 2008·Natural Product Reports·Otto Sticher
Mar 26, 2008·Journal of Medicinal Food·Ted WilsonFrances R Ragsdale
Mar 16, 2013·European Journal of Nutrition·Yeyi GuJoshua D Lambert
Feb 26, 2014·Nutrients·Nasiruddin KhanCristina Andres-Lacueva
Aug 9, 2013·Bioscience, Biotechnology, and Biochemistry·Koichiro SuzukiTeruo Miyazawa
Jan 8, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cheng Chia MengAmin Ismail
Jan 1, 2016·Journal of Chromatography. a·André de VilliersHarald Pasch
Dec 10, 2008·Molecular Nutrition & Food Research·Wolfgang Hümmer, Peter Schreier
Nov 22, 2008·Journal of Food Science·T WilsonN Vorsa
Apr 13, 2012·Molecular Nutrition & Food Research·Sebastian RzeppaHans-Ulrich Humpf
Nov 22, 2013·Molecular Nutrition & Food Research·Faisal AliSander Kersten
Jul 17, 2015·Oxidative Medicine and Cellular Longevity·Carole MinkerChristian D Muller
Sep 8, 2012·Food Chemistry·Agnieszka Kosińska, Wilfried Andlauer
Apr 4, 2017·Acta Biomaterialia·Daniel KulakowskiAna K Bedran-Russo

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