High-performance liquid chromatography-ultraviolet detection method for the simultaneous determination of typical biogenic amines and precursor amino acids. Applications in food chemistry

Journal of Agricultural and Food Chemistry
E MazzuccoM C Gennaro

Abstract

A reversed-phase high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination in food of biogenic amines and their precursor amino acids after a precolumn derivatization with dansyl chloride. The chromatographic conditions, selected to be suitable for mass spectrometry detection, were optimized through experimental design and artificial neural networks. The HPLC-UV method was validated by comparing the separation results with those obtained through a HPLC method, working under the same chromatographic conditions but employing mass spectrometry detection. The HPLC-UV method was then applied to the analysis of different food samples, namely, cheese, clams, salami, and beer. For all of the matrices, recoveries (relative standard deviation always <5%) always >92% were obtained. The results are discussed as a function of the total biogenic amine content and of the concentration ratio between amines and precursor amino acids.

References

Apr 1, 1996·International Journal of Food Microbiology·M H Silla Santos
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Citations

Jul 31, 2012·Analytical and Bioanalytical Chemistry·Fabio GosettiEmilio Marengo
Nov 14, 2013·Analytical and Bioanalytical Chemistry·Pratima Labroo, Yue Cui
Mar 7, 2012·Journal of Agricultural and Food Chemistry·Christine M Mayr, Peter Schieberle
Apr 3, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Donatella RestucciaNevio Picci
Jan 13, 2015·Food Chemistry·Carlo Ignazio Giovanni TuberosoStefano Mameli
Aug 25, 2016·Journal of Analytical Methods in Chemistry·U Gianfranco SpizzirriDonatella Restuccia
Sep 18, 2016·Analytica Chimica Acta·José Luis OrdóñezRaquel Maria Callejón
Dec 13, 2017·Electrophoresis·Renata Corrêa de CarvalhoFlávia Ferreira de Carvalho Marques
May 8, 2020·Journal of Analytical Methods in Chemistry·Muhammad Abdurrahman Munir, Khairiahi Haji Badri
Jan 23, 2021·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Ioanna Kosma, Anastasia Badeka
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shusuke UekusaTakeshi Fukushima

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