PMID: 9421112Jan 8, 1998Paper

High-performance liquid chromatography quantitation of plasma lamotrigine concentrations: application measuring trough concentrations in patients with epilepsy

Therapeutic Drug Monitoring
B C Sallustio, R G Morris

Abstract

Lamotrigine is a phenyltriazine anticonvulsant recently approved for clinical use. A high-performance liquid chromatographic (HPLC) method was developed using a silica column (5 microm) with an aqueous methanol mobile phase consisting of 94% methanol, 5.92% water, and 0.08% NH4H2PO4 adjusted to a final apparent pH of 4.0 and pumped at a flow rate of 1.0 ml/minute. Ultraviolet detection was carried out at a wavelength of 280 nm, and plasma samples were prepared for HPLC analysis by extraction into ethyl acetate after basification. Retention times for lamotrigine and its internal standard (BWA725C) were 10.3 and 11.2 minutes, respectively, and there was no chromatographic interference from other commonly coadministered anticonvulsants. Calibration curves were linear over a concentration range of 0.5 to 30 mg/l, with intra-assay and interassay coefficients of variation less than 8%. Assessment of assay performance in an international quality assurance program showed an average bias of 0.3% compared with the consensus mean. A review of 52 patient specimens showed that, if patients were grouped according to coadministered anticonvulsants, a significant correlation between lamotrigine dosage and concentration was evident in those coa...Continue Reading

References

Jun 6, 1992·Lancet·M J Brodie
Jan 1, 1991·Epilepsia·A W Peck
Nov 1, 1987·Clinical Pharmacology and Therapeutics·A F CohenA W Peck
Apr 1, 1995·Therapeutic Drug Monitoring·A D FraserP R Camfield
Aug 1, 1994·Journal of Neurology, Neurosurgery, and Psychiatry·I StolarekM J Brodie
Dec 1, 1993·Clinical Pharmacokinetics·B Rambeck, P Wolf
Mar 1, 1994·Epilepsia·E SchlumbergerO Dulac
May 1, 1993·Journal of Neurology, Neurosurgery, and Psychiatry·G J SchapelG Davies

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Citations

Apr 15, 2010·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Woonhyoung LeeKyoung Heo
Oct 28, 1999·International Journal of Pharmaceutics·D S AshtonK Valko
Feb 17, 2005·Electrophoresis·Vincenzo Pucci, Maria Augusta Raggi
Apr 17, 2003·European Journal of Clinical Pharmacology·Robert HermannMathias Locher
Aug 13, 2008·Therapeutic Drug Monitoring·Ian S Westley, Raymond G Morris
Jan 22, 2002·Therapeutic Drug Monitoring·V ChanS E Tett
Dec 23, 1998·British Journal of Clinical Pharmacology·R G MorrisB C Sallustio
Mar 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniel F Chollet

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