High-performance liquid chromatography/electrospray ionization ion-trap tandem mass spectrometric analysis and quantification of phosphatidylcholine molecular species in the serum of cystic fibrosis subjects supplemented with docosahexaenoic acid

Rapid Communications in Mass Spectrometry : RCM
Deborah PacettiNatale G Frega

Abstract

Since phosphatidylcholine (PC) is the most abundant phospholipid (PL) class in human serum, its concentration represents an important marker for the evaluation of lipid absorption and metabolism. High-performance liquid chromatography coupled on-line with electrospray ionization ion-trap tandem mass spectrometry (HPLC/ESI-MS/MS) was successfully applied to the quantitative analysis of PC molecular species from serum of cystic fibrosis (CF) subjects before and after supplementation with docosahexaenoic acid (DHA). Seven molecular species of PC (containing C16:0/C20:4, C16:0/C22:6, C18:0/C20:4, C18:0/C22:6, C16:0/C18:1, C16:0/C18:2 and C18:0/C18:2, respectively) were quantified using MS in the negative scan mode with 1,2-diundecanoyl-sn-glycero-phosphocholine as the internal standard. The molecular species containing DHA, C16:0/C22:6 and C18:0/C22:6, increased from 41.3 +/- 31.7 and 33.1 +/- 18.2 to 85.4 +/- 20.4 and 52.1 +/- 20.7 microg/mL serum, respectively, after a 3-month supplementation. Interestingly, the species containing arachidonic acid (C18:0/C20:4 and C16:0/C20:4) decreased from 115 +/- 55 and 139 +/- 57 to 58.1 +/- 22.5 and 70.5 +/- 28.1, respectively. HPLC/ESI-MS/MS allowed the direct analysis of the lipid extract ...Continue Reading

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Citations

May 24, 2008·Annual Review of Nutrition·Sheila M Innis, A George F Davidson
Oct 24, 2009·Pediatric Research·Luis Aldámiz-EchevarríaJuan Rodríguez-Soriano
Feb 24, 2007·Clinical Biochemistry·Thierry Charles CosteTeresinha Leal
Sep 3, 2005·Biomedical Chromatography : BMC·Brianna L Peterson, Brian S Cummings
Apr 11, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Akihiro TakateraMasafumi Matsuo
Feb 6, 2015·Journal of the American Society for Mass Spectrometry·Joshua D GilbertScott A McLuckey
Feb 20, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Oskar L KnittelfelderGerald N Rechberger
Feb 13, 2009·Current Opinion in Clinical Nutrition and Metabolic Care·Tilla S Worgall
Dec 20, 2017·The Journal of Nutritional Biochemistry·Petr ŽáčekMatthew Picklo

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