High-performance liquid chromatography/inductively coupled plasma mass spectrometry and tandem mass spectrometry for the detection of carbon-containing compounds

Rapid Communications in Mass Spectrometry : RCM
Christopher SmithDavid Crowther

Abstract

High-performance liquid chromatography (HPLC) combined with inductively coupled plasma mass spectrometry (ICPMS) has been studied as a means for the detection of carbon to provide a 'universal' method for detecting organic compounds in chromatographic eluents. Carbon is particularly difficult to ionise and the amount of carbon present in normal chromatographic systems leads to high backgrounds, making detection a challenge. Novel separation approaches were therefore employed, using either entirely aqueous eluents (at temperatures of 60 and 160 degrees C, dependent on the column used) to eliminate the organic modifier completely, or isotopically enriched solvents. For the aqueous eluents, detection limits for sulphanilamide were found to be 2.26 microg, corresponding to 1.13 micromol (0.47 micromol of carbon), injected on a conventional 4.6 mm i.d. column. The use of a narrow bore column with highly isotopically enriched 12C-methanol (99.95 atom%) as organic modifier for the mobile phase enabled the detection of 86 micromol for 13C-triple-labelled caffeine and 79 micromol for 13C-double-labelled phenacetin. The sensitive detection of 12C-compounds with 13C-enriched methanol as organic modifier proved impractical due to a lower l...Continue Reading

Citations

Oct 20, 2011·Analytical and Bioanalytical Chemistry·Sergio Cueto DíazJosé Ignacio García Alonso
Apr 7, 2012·Analytical and Bioanalytical Chemistry·Björn Meermann, Michael Sperling
Jul 1, 2009·Bioanalysis·Pat WrightBarclay Shilliday
Mar 26, 2011·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Pat Wright
Sep 14, 2007·Mass Spectrometry Reviews·Jean-Philippe GodinGérard Hopfgartner
Nov 4, 2004·Journal of Pharmaceutical and Biomedical Analysis·Orapin ChienthavornEva M Lenz
Sep 17, 2015·Journal of Agricultural and Food Chemistry·Daobing WangZhanbin Huang
Nov 8, 2005·Mass Spectrometry Reviews·Ryszard LobińskiJoanna Szpunar

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