High-performance size-exclusion chromatographic procedure for the determination of fluoresceinyl isothiocyanate dextrans of various molecular masses in biological media.

Journal of Chromatography
P KurtzhalsM Johansen

Abstract

A high-performance size-exclusion chromatographic procedure, using Nucleosil Diol, for the quantitative analysis of fluoresceinyl isothiocyanate dextrans of various molecular masses (10,000-150,000) in biological media was developed. The influence of the molecular mass and the degree of substitution of the conjugates on the chromatographic behaviour are discussed. In addition to quantitation, the molecular mass of the conjugates with degree of substitution below 1.6 could be estimated from the chromatograms. Linear standard calibration curves were obtained at concentrations down to 0.050 micrograms ml-1 in rabbit plasma and urine and homogenates of rabbit liver, lymph node and muscle when the derivative (degree of substitution 0.85) was monitored by fluorescence detection (lambda ex = 493 nm, lambda em = 520 mm). The fluoresceinyl isothiocyanate dextrans were found to be stable for more than three days at 37 degrees in all biological media under investigation. A pH-rate profile for the alkaline hydrolysis of fluoresceinyl isothiocyanate dextrans was constructed. The applicability of the method to pharmacokinetic studies was demonstrated by recording the plasma concentration-time profile of a fluoresceinyl isothiocyanate dextran...Continue Reading

Citations

Jan 12, 2007·The Protein Journal·Sweta Parikh, Bal Ram Singh
Oct 6, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·R Mehvar
Jul 30, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·H M NielsenM R Rassing
Sep 19, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·R Mehvar
Jun 27, 2000·International Journal of Pharmaceutics·Y KaneoT Uemura
Mar 9, 2013·Journal of Materials Science. Materials in Medicine·F RossoR V Iaffaioli
Jun 1, 1990·Analytical Chemistry·H G Barth, B E Boyes
Jan 18, 2006·Drug Metabolism and Pharmacokinetics·Yoshiharu KaneoYuka Ikeda
Apr 3, 2020·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yoshiharu Kaneo
Oct 31, 2002·Analytical Chemistry·Karine AndrieuxCécile Grabielle-Madelmont
Feb 1, 1996·Journal of Bacteriology·P Demchick, A L Koch

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