High-pH anion-exchange chromatography with pulsed amperometric detection and molar response factors of human milk oligosaccharides

Journal of Chromatography. B, Biomedical Applications
C KunzH Egge

Abstract

A method is described to separate and characterize neutral and acidic lactose-derived oligosaccharides without prior derivatization or reduction by high-pH anion-exchange chromatography and pulsed amperometric detection (HPAEC-PAD). This method has been applied to human milk oligosaccharides from donors with different blood group specificity (A, Le(a) and A, Le(b). Neutral and acidic components were separated from each other by anion-exchange chromatography. A distinct separation of individual components was obtained by size-exclusion chromatography on Fractogel TSK HW 50S (acidic oligosaccharides) or Fractogel TSK HW 40S (neutral oligosaccharides containing up to 6 monomers) and Bio-Gel P-4 size exclusion (neutral oligosaccharides containing more than 6 monomers). Furthermore the moral response factors after HPAEC-PAD have been determined for 28 components.

References

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Citations

Sep 6, 2011·The British Journal of Nutrition·Silvia RudloffClemens Kunz
Jan 24, 2012·The British Journal of Nutrition·Evelyn Jantscher-KrennLars Bode
Jul 21, 2000·Isotopes in Environmental and Health Studies·S ObermeierC Kunz
Aug 15, 2000·Annual Review of Nutrition·C KunzS Strobel
Dec 17, 2009·PloS One·Peter I DuncanNorbert Sprenger
May 16, 2012·Advances in Nutrition·G Adrienne Weiss, Thierry Hennet
May 16, 2012·Advances in Nutrition·Clemens Kunz
Dec 2, 2011·Annual Review of Food Science and Technology·Maciej ChichlowskiDavid A Mills
Aug 25, 2012·BMB Reports·Kyunghun JeongJaehan Kim
Jun 11, 2014·Beneficial Microbes·S MusilovaV Bunesova
Feb 16, 2000·The British Journal of Nutrition·C KunzD Braun
Sep 8, 2016·Journal of Pediatric Gastroenterology and Nutrition·Clemens KunzSilvia Rudloff
Oct 21, 2017·Nutrition Reviews·Stephan ThurlBernd Stahl
Jun 3, 2008·Electrophoresis·Yuanwu Bao, David S Newburg
Sep 29, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thomas FischöderLothar Elling
Mar 6, 1999·Journal of Pediatric Gastroenterology and Nutrition·A SchwertmannC Kunz
Jan 24, 2016·Electrophoresis·Veronica MantovaniNicola Volpi
Oct 14, 2017·Analytical and Bioanalytical Chemistry·Matthias MechelkePetra Kornberger
Dec 9, 2000·The Journal of Nutrition·M J GnothS Rudloff
Nov 9, 2019·Nutrients·Sander S van Leeuwen
May 22, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Neil RumachikYan Liu
Feb 8, 2014·Analytical Chemistry·Qiuting HongCarlito B Lebrilla
Jul 28, 2011·Journal of Agricultural and Food Chemistry·Bertram FongPaul McJarrow
Mar 29, 2001·Journal of the American Chemical Society·H M KimS J Danishefsky

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