High phenol degradation capacity of a newly characterized Acinetobacter sp. SA01: Bacterial cell viability and membrane impairment in respect to the phenol toxicity

Ecotoxicology and Environmental Safety
Shahab ShahryariKambiz Akbari Noghabi

Abstract

An efficient phenol-degrading bacterial strain, belonging to Acinetobacter genus, was isolated and selected to study the impact of different environmentally relevant phenol concentrations on the degradation process. The bacterial isolate, labeled as Acinetobacter sp. SA01 was able to degrade the maximum phenol concentration of 1 g/l during 60 h at optimum condition of pH 7, 30 °C and 180 rpm. Aeration and initial cell density, the two important factors, were carefully examined in the optimal growth conditions. The results showed that these two variables related proportionally with phenol degradation rate. Further investigations showed no effect of inoculum size on the enhancement of degradation of phenol at over 1 g/l. Flow cytometry (FCM) study was performed to find out the relationship between phenol-induced damages and phenol degradation process. Single staining using propidium iodide (PI) showed increased cell membrane permeability with an increase of phenol concentration, while single staining with carboxyfluorescein diacetate (cFDA) demonstrated a considerable reduction in esterase activity of the cells treated with phenol at more than 1 g/l. A detailed investigation of cellular viability using concurrent double staining ...Continue Reading

Citations

May 6, 2019·Journal of Industrial Microbiology & Biotechnology·Seth Van DexterRaj Boopathy
Aug 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yujuan WenYuesuo Yang
Jul 8, 2021·Applied and Environmental Microbiology·Mengqi XieJianrong Chen
Nov 25, 2019··力 贾建明 陈

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